The morphologies of breast cancer cell lines in three‐dimensional assays correlate with their profiles of gene expression

Molecular Oncology - Tập 1 Số 1 - Trang 84-96 - 2007
Paraic A. Kenny1,2, Genee Y. Lee1,2, Connie A. Myers1,3, Richard M. Neve1, Jeremy R. Semeiks1, Paul T. Spellman1, Katrin Lorenz1,4, Eva H. Lee1, Mary Helen Barcellos‐Hoff1, Ole W. Petersen5, Joe W. Gray1, Mina J. Bissell1
1Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, One Cyclotron Road, MS 977-225A, Berkeley, CA 94720, USA
2These authors contributed equally to this report.
3Present address: Division of Neuropathology, Washington University School of Medicine, St. Louis, MO 63110, USA.
4Present address: Department of Molecular Medicine, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.
5Structural Cell Biology Unit, Institute of Medical Anatomy, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen N, Denmark

Tóm tắt

Abstract3D cell cultures are rapidly becoming the method of choice for the physiologically relevant modeling of many aspects of non‐malignant and malignant cell behavior ex vivo. Nevertheless, only a limited number of distinct cell types have been evaluated in this assay to date. Here we report the first large scale comparison of the transcriptional profiles and 3D cell culture phenotypes of a substantial panel of human breast cancer cell lines. Each cell line adopts a colony morphology of one of four main classes in 3D culture. These morphologies reflect, at least in part, the underlying gene expression profile and protein expression patterns of the cell lines, and distinct morphologies were also associated with tumor cell invasiveness and with cell lines originating from metastases. We further demonstrate that consistent differences in genes encoding signal transduction proteins emerge when even tumor cells are cultured in 3D microenvironments.

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Molecular Oncology

Tập 1 Số 1

84-96

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