Sự phân bố và lan truyền toàn cầu của gen kháng colistin mcr-1 đã được di động hóa

Nature Communications - Tập 9 Số 1
Ruobing Wang1, Lucy van Dorp2, Liam P. Shaw2, Phelim Bradley3, Qi Wang1, Xiaojuan Wang1, Longyang Jin1, Qing Zhang4, Yuqing Liu4, Adrien Rieux5, Thamarai Schneiders6, Lucy A. Weinert7, Zamin Iqbal8, Xavier Didelot9, Hui Wang1, François Balloux2
1Department of Clinical Laboratory, Peking University People's Hospital, Beijing 100044, China
2UCL Genetics Institute, University College London, Gower Street, London WC1E 6BT, UK
3Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK
4Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Shandong Province, Jinan, 250100, China
5UMR PVBMT, CIRAD, 97410, St Pierre, Reunion, France
6Division of Infection and Pathway Medicine, 49 Little France Crescent, Edinburgh, EH16 4SB, UK
7Department of Veterinary Medicine, Cambridge, CB3 0ES, UK
8European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Cambridge CB10 1SD, UK
9Department of Infectious Disease Epidemiology, Imperial College London, Norfolk Place 21, London, W2 1PG, UK

Tóm tắt

Tóm tắt

Colistin là một trong số ít loại thuốc hiện có để điều trị các nhiễm trùng do các chủng vi khuẩn Enterobacteriaceae kháng carbapenem gây ra. Do đó, sự lan truyền qua plasmid của gen kháng colistin mcr-1 gần đây tạo ra một mối đe dọa đáng kể đối với sức khỏe cộng đồng, đòi hỏi sự giám sát và theo dõi toàn cầu. Trong nghiên cứu này, chúng tôi mô tả đặc điểm sự phân bố toàn cầu của mcr-1 bằng cách sử dụng một tập dữ liệu gồm 457 trình tự mẫu mcr-1-dương tính. Chúng tôi tìm thấy mcr-1 trong các loại plasmid khác nhau nhưng xác định được một nền chung ngay lập tức cho tất cả các trình tự mcr-1. Phân tích của chúng tôi thiết lập rằng tất cả các yếu tố mcr-1 trong lưu hành đều có nguồn gốc từ sự huy động ban đầu của mcr-1 bởi một transposon ISApl1 vào giữa những năm 2000 (2002–2008; 95% mật độ hậu nghiệm cao nhất), sau đó là sự mở rộng dân số đáng kể, dẫn đến sự phân bố toàn cầu hiện nay. Kết quả của chúng tôi cung cấp phân tích phát sinh chủng loại hệ thống đầu tiên về nguồn gốc và sự lan truyền của mcr-1, và nhấn mạnh tầm quan trọng của việc hiểu sự di chuyển của các gen kháng sinh khắp các cấp độ tổ chức hệ gen khác nhau.

Từ khóa

#plasmid-mediated resistance #colistin #mcr-1 gene #antibiotic resistance #public health threat #global monitoring #genomic analysis #Enterobacteriaceae

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