The fine structure of the monkey (Macaca) sertoli cell and its role in maintaining the blood‐testis barrier

Wiley - Tập 175 Số 4 - Trang 639-656 - 1973
Martin Dym1
1Department of Anatomy, and Laboratory of Human Reproduction Reproductive Biology, Harvard Medical School, Boston, Massachusetts 02115

Tóm tắt

AbstractThe monkey Sertoli cell, a tall columnar cell, extends from the basement membrane of the seminiferous epithelium to the tubule lumen. Its nucleus occupies a basal position and reveals extensive nuclear envelope infoldings. A zone of fine filaments, approximately 0.5 μ in thickness, invests the nucleus and appears to prevent other cell organelles from approaching it. The basal cytoplasm is characterized by numerous mitochondria and abundant smooth endoplasmic reticulum. Lipid droplets, 3 to 4 μ in diameter, membrane‐limited dense bodies of various shapes and densities, Golgi cisternae, scattered free ribosomes and parallel profiles of rough endoplasmic reticulum are common. The more apical portions of the cell contain longitudinally oriented microtubules and rod‐shaped mitochondria, but other organelles are rare.The seminiferous tubules of monkeys are surrounded by three to five circumferentially arranged cells that overlap each other but are separated by intercellular spaces of at least 300 to 400 Å. Tracers such as horseradish peroxidase and lanthanum nitrate injected intravascularly readily pass between the peritubular cells and enter the germinal epithelium. Within the epithelium the tracers outline the spermatogonia and early spermatocytes by permeating the surrounding intercellular spaces. Further penetration toward the tubule lumen is effectively prevented by the occluding tight junctions joining adjacent Sertoli cells. Thus, in monkeys the peritubular epithelioid cells do not impede vascularly introduced tracers from penetrating into the germinal epithelium. The only morphological component of the blood‐testis barrier in the macaque appears to be the Sertoli‐Sertoli occluding junction.

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Tài liệu tham khảo

10.1530/jrf.0.0280299

Barr A.1967Human spermatogenesis. M. Sc. thesis McGill University.

Bennett W. I.1971Unpublished observations.

Blackshaw A. W., 1970, The Testis, 73, 10.1016/B978-0-12-386602-8.50007-5

Brown H. H., 1885, On spermatogenesis in the rat, Quart. J. of Micro. Sci., 25, 343

Carr I., 1968, Sertoli cells as phagocytes: an electron microscopic study, J. Anat., 102, 501

10.1083/jcb.26.3.911

10.1210/endo-76-4-646

10.1016/0014-4827(58)90052-1

10.1002/aja.1001110202

10.1002/aja.1001260106

10.1002/aja.1001220205

Davis J. R., 1970, The Testis, 259, 10.1016/B978-0-12-386602-8.50010-5

10.1002/jmor.1051200402

Dym M., 1972, The fine structure of the monkey Sertoli cell and its role in establishing the blood‐testis barrier, Biol. of Reprod., 7, 129

10.1002/aja.1001280302

10.1093/biolreprod/3.3.308

Eddy E. M. andA. I.Kahri1971Unpublished observations.

10.1002/ar.1091060306

10.1002/aja.1001130104

Fawcett D. W. andS.Ito1972Unpublished observations.

Fawcett D. W., 1970, Electron microscopic observations on the structural components of the blood‐testis barrier, J. Reprod. Fertil., 10, 105

Fawcett D. W. W. B.NeavesandM. N.Flores1973Comparative observations of intertubular lymphatics and the organization of the interstitial tissue of the mammalian testis.Biol. of Reprod. in press.

Fawcett D. W., 1969, Observations on the release of spermatozoa and on changes in the head during passage through the epididymis, J. Reprod. Fertil. Suppl., 6, 405

10.1177/14.4.291

10.1210/endo-84-3-488

Karnovsky M. J., 1965, A formaldehyde‐glutaraldehyde fixative of high osmolarity for use in electron microscopy, J. Cell Biol., 27, 137a

10.1007/BF00305816

10.1093/oxfordjournals.bmb.a070748

Lacy D., 1968, The Sertoli cell and spermatogenesis in mammals, 1019

10.1210/endo-91-1-147

Niemi M., 1965, Cyclical changes in and significance of lipids and acid phosphatase activity in the seminiferous tubules of the rat testis, Anat, Rec., 151, 159

10.1508/cytologia.19.203

10.1002/aja.1000990303

10.1002/ar.1091740404

Reddy J. N., 1967, Peroxidase transport by Sertoli cells of the rat testis, J. Cell Biol., 35, 184a

Regaud C., 1900, Les phases et les stades de ľonde spermatogènétique chez les mammifères (rat). Classification rationnelle des figures de la spermatogenéses, Comptes Rendus Soc. de Biol., 52, 1039

Regaud C., 1901, Etudes sur la structure des tubes séminifères et sur la spermatogenèse chez les mammifères, Arch. Anat. Micro, 4, 101

10.1007/BF00360605

10.1007/BF00340602

10.1071/ZO9690729

Sertoli E., 1865, Dell 'esistenza di particolari cellule ramificate nei canalicoli seminiferi del testiculo umano, Morgagni, 7, 31

Setchell B. P., 1967, The blood‐testicular fluid barrier in sheep, J. Physiol., 189, 63p

10.1007/BF00593663

Waites G. M. H., 1969, The gonads, 649

von Winiwarter H., 1912, Etudes sur la spermatogenèse humaine. I. Cellule de Sertoli. II Hétérochromosome et mitoses de ľépithélium séminal, Arch de Biol., 27, 91

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Wiley

Tập 175 Số 4

639-656

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