The efficacy of antioxidants in functional recovery of spinal cord injured rats: an experimental study
Tóm tắt
A total of 30 female Sprague-Dawley rats (180–220 g) subjected to spinal cord injury (SCI) were divided into three groups of ten rats each. Group 1 served as control (SCI + Saline), Group 2 received daily dose of ascorbic acid 2,000 mg/kg body weight and group 3 rats received alpha tocopherol daily with the dose of 2,000 mg/kg body weight for 14 days. The Spontaneous coordinate activity (SCA), Basso, Beattie, and Bresnahan (BBB) and Tarlov locomotor scores were used to assess functional recovery of SCI rats. Compared to group 1, group 2 showed statistically insignificant improvement in the SCA, BBB and Tarlov scores at the end of the study. Compared to group 1, group 3 showed statistically significant improvement in the SCA (P < 0.001), BBB (P < 0.001) and Tarlov (P < 0.01) scores at the end of the study. In conclusion, the administration of alpha-tocopherol enhances the reparative effects against SCI and it is more effective than ascorbic acid.
Tài liệu tham khảo
Dumont RJ, Verma S, Okonkwo DO, Hurlbert RJ, Boulos PT, Ellegala DB et al (2001) Acute spinal cord injury, part II: contemporary pharmacotherapy. Clin Neuropharmacol 24:265–279
Diaz-Ruiz A, Alcaraz-Zubeldia M, Maldonado V, Salgado-Ceballos H, Mendez-Armenta M, Rios C (2009) Differential time-course of the increase of antioxidant thiol-defenses in the acute phase after spinal cord injury in rats. Neurosci Lett 452:56–59
Kochanowski J, Malara A, Chudzik W, Kaczorowska B (1999) Pathophysiology and treatment of spinal cord medulla injuries. Neurol Neurochir Pol 32(Suppl 6):91–97
Hausmann ON (2003) Post-traumatic inflammation following spinal cord injury. Spinal Cord 41:369–378
Wells JE, Rice TK, Nuttall RK et al (2003) An adverse role for matrix metalloproteinase 12 after spinal cord injury in mice. J Neurosci 23:10107–10115
Hall ED (2001) Pharmacological treatment of acute spinal cord injury: how do we build on past success? J Spinal Cord Med 24:142–146
Bethea JR, Dietrich WD (2002) Targeting the host inflammatory response in traumatic spinal cord injury. Curr Opin Neurol 15:355–360
Soares S, Barnat M, Salim C (2007) Extensive structural remodeling of the injured spinal cord revealed by phosphorylated MAP1B in sprouting axons and degenerating neurons. Eur J Neurosci 26:1446–1461
Lynskey JV, Sandhu FA, Dai HN et al (2006) Delayed intervention with transplants and neurotrophic factors supports recovery of forelimb function after cervical spinal cord injury in adult rats. J Neurotrauma 23:617–634
Li Y, Raisman G (1994) Schwann cells induce sprouting in motor and sensory axons in the adult rat spinal cord. J Neurosci 14:4050–4063
Ramon-Cueto A, Cordero MI, Santos-Benito FF et al (2000) Functional recovery of paraplegic rats and motor axon regeneration in their spinal cords by olfactory ensheathing glia. Neuron 25:425–435
Xu XM, Guenard V, Kleitman N (1995) Axonal regeneration into Schwann cell-seeded guidance channels grafted into transected adult rat spinal cord. J Comp Neurol 351:145–160
Onifer SM, Nunn CD, Decker JA et al (2007) Loss and spontaneous recovery of forelimb evoked potentials in both the adult rat cuneate nucleus and somatosensory cortex following contusive cervical spinal cord injury. Exp Neurol 207:238–247
Hao JX, Xu XJ (2003) Animal models of spinal cord injury pain and their implications for pharmacological treatments. J Rehabil Med (41 Suppl):81–84
Blight AR, Zimber MP (2001) Acute spinal cord injury: pharmacotherapy and drug development perspectives. Curr Opin Investig Drugs 2:801–808
Halliwell B, Gutteridge JM (1984) Free radicals, lipid peroxidation, and cell damage. Lancet 2:1095
Suzuki J, Abiko H, Mizoi K, Oba M, Yoshimoto T (1987) Protective effect of phenytoin and its enhanced action by combined administration with mannitol and vitamin E in cerebral ischaemia. Acta Neurochir (Wien) 88:56–64
Al Jadid MS, Robert A, Al-Mubarak S (2009) The efficacy of alpha-tocopherol in functional recovery of spinal cord injured rats: an experimental study. Spinal Cord 47:662–667
Basso DM, Beattie MS, Bresnahan JC (1995) A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma 12:1–21
Tarlov IM (1957) Spinal cord compression. In: Mechanism of paralysis and treatment. Charles C. Thomas, Springfield, P 147
Wells JE, Hurlbert RJ, Fehlings MG, Yong VW (2003) Neuroprotection by minocycline facilitates significant recovery from spinal cord injury in mice. Brain 126:1628–1637
Lemke M, Frei B, Ames BN, Faden AI (1990) Decreases in tissue levels of ubiquinol-9 and -10, ascorbate and alpha-tocopherol following spinal cord impact trauma in rats. Neurosci Lett 108:201–206
Landry E, Frenette J, Guertin PA (2004) Body weight, limb size, and muscular properties of early paraplegic mice. J Neurotrauma 21:1008–1016
Morse L, Teng YD, Pham L, Newton K, Yu D, Liao WL et al (2008) Spinal cord injury causes rapid osteoclastic resorption and growth plate abnormalities in growing rats (SCI-induced bone loss in growing rats). Osteoporos Int 19:645–652
Primeaux SD, Tong M, Holmes GM (2007) Effects of chronic spinal cord injury on body weight and body composition in rats fed a standard chow diet. Am J Physiol Regul Integr Comp Physiol 293:R1102–R1109
Liao JW, Song YM (2004) Preliminary study of the effects of high-dose Vitamin C on acute spinal cord injury in rats. Sichuan Da Xue Xue Bao Yi Xue Ban 35:854–857
Iwasa K, Ikata T (1988) An experimental study on preventive effect of vitamin E in spinal cord injury. Nippon Seikeigeka Gakkai Zasshi 62:767–775
Hall ED, Braughler JM, McCall JM (1992) Antioxidant effects in brain and spinal cord injury. J Neurotrauma Suppl 1:S165–S172
Katoh D, Ikata T, Katoh S, Hamada Y, Fukuzawa K (1996) Effect of dietary vitamin C on compression injury of the spinal cord in a rat mutant unable to synthesize ascorbic acid and its correlation with that of vitamin E. Spinal Cord 34:234–238
Cristante AF, Barros Filho TE, Oliveira RP, Marcon RM, Rocha ID, Hanania FR et al (2009) Antioxidative therapy in contusion spinal cord injury. Spinal Cord 47:458–463
Logan MP, Parker S, Shi R (2005) Glutathione and ascorbic acid enhance recovery of Guinea pig spinal cord white matter following ischemia and acrolein exposure. Pathobiology 72:171–178