The effects of acute doxorubicin treatment on proteome lysine acetylation status and apical caspases in skeletal muscle of fasted animals

Journal of Cachexia, Sarcopenia and Muscle - Tập 4 Số 3 - Trang 239-243 - 2013
Amie J. Dirks‐Naylor1, Ngan T.K. Tran1, Sendra Yang1, Raean Mabolo1, Samir A. Kouzi1
1School of Pharmacy, Wingate University, 515 N. Main Street, 28174 Wingate, NC

Tóm tắt

BackgroundDoxorubicin treatment is known to cause muscular weakness. However, the cellular mechanisms have not been elucidated. We aimed to determine the effects of acute doxorubicin treatment on proteome lysine acetylation status, an indication of the apoptotic and inflammatory environment, and the expression and activation of various apical caspases involved in the initiation of apoptosis.MethodsSix‐week‐old male F344 rats were injected intraperitoneally with 20 mg/kg of doxorubicin or saline. Once the treatment was administered, both groups of animals were fasted with no food or water until sacrifice 24 h posttreatment.ResultsDoxorubicin treatment affected neither the proteome lysine acetylation status nor the expression of sirtuin 1, sirtuin 3, SOD1, or SOD2 in soleus of fasted animals. Doxorubicin treatment also did not affect the expression or activation of procaspase‐1, procaspase‐8, procaspase‐9, or procaspase−12.ConclusionWe suggest that doxorubicin does not exert a direct effect on these catabolic parameters in skeletal muscle in vivo.

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Thông tin
Thông tin xuất bản

Journal of Cachexia, Sarcopenia and Muscle

Tập 4 Số 3

239-243

Thông tin tác giả