The effects of PRGF on bone regeneration and on titanium implant osseointegration in goats: A histologic and histomorphometric study

Journal of Biomedical Materials Research - Part A - Tập 91A Số 1 - Trang 158-165 - 2009
Eduardo Anitua1, Gorka Orive1, Rafael Plá2, Pedro Amigo Román3, Victoriano Serrano4, Isabel Andı́a1
1Biotechnology Institute I MAS D, San Antonio 15, 01005 Vitoria, Spain
2Private Practice in Dentistry, Albacete, Spain
3Private Practice in Dentistry, Elche, Spain
4University Complutense of Madrid, Spain

Tóm tắt

AbstractThe effect of local application of scaffold‐like preparation rich in growth factors (PRGF) on bone regeneration in artificial defects and the potential effect of humidifying titanium dental implants with liquid PRGF on their osseointegration were investigated. The PRGF formulations were obtained from venous blood of three goats and applied either as a 3D fibrin scaffold (scaffold‐like PRGF) in the regeneration of artificial defects or as liquid PRGF via humidifying the implants before their insertion. Initially, 12 defects were filled with scaffold‐like PRGF and another 12 were used as controls. The histological analysis at 8 weeks revealed mature bone trabeculae when PRGF was used, whereas the control samples showed mainly connective tissue with incipient signs of bone formation. For the second set of experiments, 26 implants (13 humidified with liquid PRGF) were placed in the tibiae of goats. Histological and histomorphometric results demonstrated that application of liquid PRGF increased the percentage of bone‐implant contact in 84.7%. The whole surface of the PRGF‐treated implants was covered by newly formed bone, whereas only the upper half was surrounded in control implants. In summary, PRGF can accelerate bone regeneration in artificial defects and improve the osseointegration of titanium dental implants. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009

Từ khóa


Tài liệu tham khảo

10.1152/physrev.2003.83.3.835

10.2106/00004623-200206000-00022

10.1016/j.ejpb.2004.03.004

10.1160/TH03-07-0440

Anitua E, 1999, Plasma rich in growth factors: Preliminary results of the use in the preparation of future sites for implants, Int J Oral Maxillofac Implants, 14, 529

10.1016/S1079-2104(98)90029-4

10.1016/S0142-9612(03)00114-5

10.1111/j.1423-0410.2006.00815.x

10.1016/j.jebdp.2006.04.018

10.1016/j.tibtech.2006.02.010

Aghaloo TL, 2004, Evaluation of platelet rich plasma in combination with anorganic bovine bone in the rabbit cranium: A pilot study, Int J Oral Maxillofac Implants, 19, 59

10.1111/j.1600-0501.2005.01208.x

10.1002/jbm.a.30585

10.1016/j.biomaterials.2007.06.037

Adell R, 1990, Reconstruction of severely resorbed edentulous maxillae using osseointegrated fixtures in immediate autogenous bone grafts, Int J Oral Maxilof Implants, 5, 233

Schropp L, 2003, Bone healing and soft issue contour changes following single‐tooth extraction: A clinical and radiographic 12‐month prospective study, Int J Periodont Restor Dent, 23, 313

10.1016/S0278-2391(98)90286-5

10.1016/j.joms.2004.09.012

10.1002/jor.20278

10.1016/j.orthres.2004.08.015

10.1097/01.prs.0000255543.43695.1d

10.3109/03008208909002419

10.1038/sj.emboj.7600067

10.1038/nm0603-669

Suba Z, 2004, Facilitation of β‐tricalcium phosphate induced alveolar bone regeneration by platelet rich plasma in beagle dogs: A histologic and histomorphometric study, Int J Oral Maxillofac Implants, 19, 832

10.1249/01.MSS.0000089344.44434.50

10.1002/jbm.a.30585

10.1111/j.1600-0501.2006.01367.x

10.1016/j.biomaterials.2006.10.020

10.1034/j.1600-0501.2002.130513.x

10.1007/s10006-002-0367-6

Doucet C, 2005, Platelet lysates promote mesenchymal stem cell expansion: A safety substitute for animal serum in cell‐based therapy applications, J Cell Physiol, 9999, 1

10.1111/j.1743-6109.2006.00110.x

10.1016/j.joen.2006.01.007

Dean JW, 1995, Fibronectin and laminin enhance gingival cell attachment to dental implant surfaces in vitro, Int J Maxillofac Implants, 10, 721

10.1006/excr.1994.1257

10.1016/S0928-4931(02)00321-1

Fuerst G, 2003, Enhanced bone‐to‐implant contact by platelet‐released growth factors in mandibular cortical bone: A histomorphometric study in minipigs, Int J Maxillofac Implants, 18, 685

10.1089/ten.2006.12.2555

Thông tin
Thông tin xuất bản

Journal of Biomedical Materials Research - Part A

Tập 91A Số 1

158-165

Thông tin tác giả