The effect of body temperature on the murine electroretinogram

Springer Science and Business Media LLC - Tập 106 - Trang 239-242 - 2003
Jian Kong1, Peter Gouras1
1Department of Ophthalmology, Columbia University, New York, USA

Tóm tắt

Purpose: To study the effect of body temperature on the murine electroretinogram (ERG). Methods: The corneal ERG elicited by a strobe flash from dark-adapted mice was recorded using a saline wick electrode while measuring rectal temperature continuously. The mouse was placed within a cylindrical coil of tubing through which water circulated from a temperature controlled bath. The body temperature of the mouse was changed stepwise between 30 and 37 °C. Results: ERGs of approximately normal configuration were recorded at body temperature ranging between 30 and 37 °C. The maximum amplitude of the a- and b-waves varied linearly with temperature. The rate of change of b-wave amplitude was about 100 μV/degree. At 30 °C, maximum b-wave amplitude was about 400 μV; at 37 °C it was about 1000 μV. A change in body temperature produced a rapid change in ERG amplitude. Conclusion: The murine ERG is very sensitive to changes in temperature. In order to monitor the ERG accurately over time, continuous recording of body temperature is essential.

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Tài liệu tham khảo

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Springer Science and Business Media LLC

Tập 106

239-242

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