The direct antiglobulin test: A critical step in the evaluation of hemolysis

American Journal of Hematology - Tập 87 Số 7 - Trang 707-709 - 2012
Nicole D. Zantek1, Scott A. Koepsell, Daryl R. Tharp, Claudia S. Cohn
1Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN, USA. [email protected]

Tóm tắt

AbstractThe direct antiglobulin test (DAT) is a laboratory test that detects immunoglobulin and/or complement on the surface of red blood cells. The utility of the DAT is to sort hemolysis into an immune or nonimmune etiology. As with all tests, DAT results must be viewed in light of clinical and other laboratory data. This review highlights the most common clinical situations where the DAT can help classify causes of hemolysis, including autoimmune hemolytic anemia, transfusion‐related hemolysis, hemolytic disease of the fetus/newborn, drug‐induced hemolytic anemia, passenger lymphocyte syndrome, and DAT‐negative hemolytic anemia. In addition, the pitfalls and limitations of the test are addressed. False reactions may occur with improper technique, including improper washing, centrifugation, and specimen agitation at the time of result interpretation. Patient factors, such as spontaneous red blood cell agglutination, may also contribute to false results. Am. J. Hematol. 87:707–709, 2012. © 2012 Wiley Periodicals, Inc.

Từ khóa


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Thông tin
Thông tin xuất bản

American Journal of Hematology

Tập 87 Số 7

707-709

Thông tin tác giả