The determination of total and unbound midazolam in human plasma. A comparison of high performance liquid chromatography, gas chromatography and gas chromatography/mass spectrometry

Biomedical Chromatography - Tập 4 Số 1 - Trang 28-33 - 1990
J. X. de Vries1, J. Rudi1, I. Walter‐Sack1, R. Conradi2
1Abteilung für Klinische Pharmakologie, Medizinische Klinik der Universität, Bergheimer Strasse 58, 6900 Heidelberg
2Klinik für Anästhesiologie der Universität, Im Neuenheimer Feld 110, 6900 Heidelberg, FRG

Tóm tắt

AbstractMidazolam concentrations in patients' plasma was determined after extraction with high performance liquid chromatography (HPLC), gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). GC was selected for routine plasma assays in terms of selectivity, simplicity, precision, accuracy and sensitivity (0.02 μg/ml); HPLC analysis was less sensitive (0.1 μg/mL) than GC; GC/MS was used for analysis validation. Plasma protein binding of midazolam was determined by GC in patients' plasma after in vitro incubation with midazolam, ultrafiltration and extraction; 5% of the drug was unbound to plasma proteins. Midazolam distribution in lipoprotein fractions separated by ultracentrifugation of plasma obtained from patients on prolonged midazolam treatment was also assayed by GC.

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Biomedical Chromatography

Tập 4 Số 1

28-33

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