The crystal structure of apo‐pseudoazurin from Alcaligenes faecalis S‐6

FEBS Letters - Tập 368 - Trang 432-434 - 1995
Kyriacos Petratos1, Maria Papadovasilaki1, Zbigniew Dauter2,3
1Institute of Molecular Biology and Biotechnology (I.M.B.B.)-F.O.R.T.H., P.O. Box 1527, 711 10 Heraklion, Greece
2European Molecular Biology Laboratory (EMBL) clo DESY, Notkestr. 85, 22603 Hamburg 52, Germany
3Department of Chemistry, University of York, Heslington, York Y01 5DD, UK

Tóm tắt

The 3D structure of the apo‐pseudoazurin (copper free pseudoazurin) from Alcaligenes faecalis strain S‐6 is determined and refined at pH 6.7 using X‐ray diffraction data to 1.85Åresolution. The final crystallographicR‐factor is 0.164. Comparing the structures of apo‐pseudoazurin and the native (Cu2+) protein, we observed limited differences ranging between 0.1–0.4Åat the vicinity of the copper site, at the loops connecting the secondary structural elements, at certain β‐strands and at the amino and car☐y termini of the protein.

Tài liệu tham khảo

10.1093/oxfordjournals.jbchem.a133221 10.1093/oxfordjournals.jbchem.a133220 10.1021/bi00183a030 10.1107/S0108768188008912 10.1016/S0021-9258(17)43220-0 10.1016/0014-5793(92)80981-L 10.1016/0162-0134(91)84154-2 10.1002/pro.5560020506 10.1016/0014-5793(94)00544-3 10.1016/0014-5793(87)81048-7 Z. Otwinowski 1991 Yale Univ New Haven CT USA Lamzin V.S., 1993, Acta Cryst., 49, 129 10.1107/S0021889878013308 Konnert J.H., 1980, Acta Cryst., 34, 791 10.1107/S0567739480001210 CCP4 1979 Daresbury Laboratory Warrington WA4 4AS UK 10.1107/S0365110X52002161 10.1038/355472a0 Vallee B., 1968, Proc. Natl. Acad. Sci. USA, 498 10.1080/02603598308078118
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FEBS Letters

Tập 368

432-434

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