The crystal structure of apo‐pseudoazurin from Alcaligenes faecalis S‐6

FEBS Letters - Tập 368 - Trang 432-434 - 1995
Kyriacos Petratos1, Maria Papadovasilaki1, Zbigniew Dauter2,3
1Institute of Molecular Biology and Biotechnology (I.M.B.B.)-F.O.R.T.H., P.O. Box 1527, 711 10 Heraklion, Greece
2European Molecular Biology Laboratory (EMBL) clo DESY, Notkestr. 85, 22603 Hamburg 52, Germany
3Department of Chemistry, University of York, Heslington, York Y01 5DD, UK

Tóm tắt

The 3D structure of the apo‐pseudoazurin (copper free pseudoazurin) from Alcaligenes faecalis strain S‐6 is determined and refined at pH 6.7 using X‐ray diffraction data to 1.85Åresolution. The final crystallographicR‐factor is 0.164. Comparing the structures of apo‐pseudoazurin and the native (Cu2+) protein, we observed limited differences ranging between 0.1–0.4Åat the vicinity of the copper site, at the loops connecting the secondary structural elements, at certain β‐strands and at the amino and car☐y termini of the protein.


Tài liệu tham khảo

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FEBS Letters

Tập 368

432-434

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