The combined action of binase and bleomycin on human lung adenocarcinoma cells
Tóm tắt
Some microbial ribonucleases (RNases) demonstrate selective cytotoxic effect against a wide range of tumor cells. In this context combined use of cytotoxic RNases in complex therapy with other chemotherapeutic agents appears to be especially promising. In this study we have investigated the apoptosisinduced effect of Bacillus pumilus RNase (binase) in combination with known antitumor antibiotic bleomycin on human lung adenocarcinoma A549 cells. The combined effect of high concentrations of these agents did not have any mutual increase in their apoptosisinduced action, while a combination of nonapoptotic concentrations resulted in the increase of proportion of apoptotic cells up to 22% as compared with individual effect of bleomycin (6%) and binase (12%) used separately. These results indicate that binase and bleomycin are effective in combination of their low concentrations and ineffective in combination of their high concentrations.
Tài liệu tham khảo
Ulyanova, V., Vershinina, V., and Ilinskaya, O., FEBS J., 2011, vol. 278, pp. 3633–3643. doi 10.1111/j. 17424658.2011.08294x
Ilinskaya, O., Decker, K., Koschinski, A., Dreyer, F., and Repp, H., Toxicology, 2001, vol. 156, pp. 101–107. doi 10.1016/S0300-483X(00)00335-8
Mitkevich, V.A., Petrushanko, I.Yu., Kretova, O.V., Spirin, P.V., Zelenikhin, P.V., Prassolov, V.S., Tchurikov, N.A., Ilinskaya, O.N., and Makarov, A.A., Cell Cycle, 2010, vol. 9, pp. 2674–2678. doi 10.4161/cc.9.13.12150
Mitkevich, V.A., Petrushanko, I.Yu., Spirin, P.V., Fedorova, T.V., Kretova, O.V., Tchurikov, N.A., Prassolov, V.S., Ilinskaya, O.N., and Makarov, A.A., Cell Cycle, 2011, vol. 10, pp. 4090–4097. doi 10.4161/cc.10.23.18210
Mitkevich, V.A., Kretova, O.V., Petrushanko, I.Y., Burnysheva, K.M., Sosin, D.V., Simonenko, O.V., Ilinskaya, O.N., Tchurikov, N.A., and Makarov, A.A., Biochimie, 2013, vol. 95, pp. 1344–1349. doi 10.1016/jbiochi.2013.02.016
Altomare, D.A., Rybak, S.M., Pei, J., Maizel, J.V., Cheung, M., Testa, J.R., and Shogen, K., BMC Cancer, 2010, vol. 10, doi 10.1186/1471-2407-10-34
Porta, C., Paglino, C., and Mutt, L., Biologics, 2008, vol. 2, pp. 601–609.
Mikulski, S.M., Newton, D.L., Wiltrout, R.H., and Rybak, S.M., Proc. Am. Assoc. Cancer Res., 1999, vol. 40, pp. 491–492.
Cabrera-Fuentes, H.A., Aslam, M., Saffarzadeh, M., Kolpakov, A., Zelenikhin, P., Preissner, K.T., and Ilinskaya, O.N., Toxicon, 2013, vol. 69, pp. 219–226. doi 10.1016/jtoxicon.2013.03.015
Abraham, A.T., Lin, J., Newton, L., Rybak, S., and Hecht, S.M., Chem. Biol., 2003, vol. 10, pp. 45–52.
Schulga, A., Kurbanov, F., Kirpichnikov, M., Protasevich, I., Lobachev, V., Ranjbar, B., Chekhov, V., Polyakov, K., Engelborghs, Y., and Makarov, A., Protein Eng., 1998, vol. 11, pp. 775–782.
Yakovlev, G.I., Moiseyev, G.P., Struminskaya, N.K., Borzykh, O.A., Kipenskaya, L.V., Znamenskaya, L.V., Leschinskaya, I.B., Chernokalskaya, E.B., and Hartley, R.W., FEBS Lett., 1994, vol. 354, pp. 305–306.
Ilinskaya, O.N., Ivanchenko, O.B., Karamova, N.S., and Kipenskaya, L.V., Mutat. Res., 1996, vol. 354, pp. 203–209.
Laakko, T., King, L., and Fraker, P., J. Immunol. Methods, 2002, vol. 261, pp. 129–139.
Burger, R.M., Chem. Rev., 1998, vol. 98, pp. 1153–1170.
Carter, B.J., de Vroom, E., Long, E.C., Van der Marel, G.A., Van der Boom, J.H., and Hecht, S.M., Proc. Natl. Acad. Sci. USA, 1990, vol. 87, pp. 9373–9377.
Lee, V.Y., Schroedl, C., Brunelle, J. K., Buccellato, L.J., Akinci, O.I., Kaneto, H., Snyder, C., Eisenbart, J., Budinger, G.R.S., and Chandel, N.S., Am. J. Physiol. Lung. Cell. Mol. Physiol., 2005, vol. 289, pp. 521–528.
Mungunsukh, O., Griffin, A.J., Lee, Y.H., and Day, R.M., Am. J. Physiol. Lung. Cell. Mol. Physiol., 2010, vol. 298, pp. 696–703. doi 10.1152/ ajplung.00322.2009
Tao, Z., Konishi, K., Keith, G., and Hecht, S.M., J. Am. Chem. Soc., 2006, vol. 128, pp. 14806–14807.
Suhasini, A.N. and Sirdeshmukh, R., J. Biol. Chem., 2006, vol. 281, pp. 12201–12209.
Makarov, A.A. and Ilinskaya, O.N., FEBS Lett., 2003, vol. 540, pp. 15–20.
Makarov, A.A., Kolchinsky, A., and Ilinskaya, O.N., BioEssays, 2008, vol. 30, pp. 781–790. doi 10.1002/bies.20789
Mitkevich, V.A., Makarov, A.A., and Ilinskaya, O.N., Mol. Biologiya 2014, vol. 48, pp. 214–219.
Thorn, C.F., Oshiro, C., Marsh, S., Hernandez-Boussard, T., McLeod, H., Klein, T.E., and Altman, R.B., Pharmacogenet. Genomics, 2011, vol. 21, pp. 440–446. doi 10.1097/FPC.0b013e32833ffb56
Majchrzak, A., Witkowska, M., Medra, A., Zwolinska, M., Bogusz, J., Cebula-Obrzut, B., Darzynkiewicz, Z., Robak, T., and Smolewski, P., Postepy Hig. Med. Dosw., 2013, vol. 67, pp. 1166–1172.