The clinical significance of MAGEA3 expression in pancreatic cancer

International Journal of Cancer - Tập 118 Số 9 - Trang 2269-2275 - 2006
Joseph Kim1,2, Howard A. Reber3, O. Joe Hines3, Kevork Kazanjian3, Andy N. Tran2, Xing Ye4, Farin Amersi1,2, Steve R. Martinez1,2, Sarah Dry5, Anton J. Bilchik1, Dave S.B. Hoon2
1Division of Surgical Oncology, John Wayne Cancer Institute, Saint John's Health Center, Santa Monica, CA, USA
2Gastrointestinal Research Section, Department of Molecular Oncology, John Wayne Cancer Institute, Saint John's Health Center, Santa Monica, CA,USA
3Section of Gastrointestinal Surgery, Department of Surgery, David Geffen School of Medicine, University of California, Los Angeles, CA, USA
4Division of Biostatistics, John Wayne Cancer Institute, Saint John's Health Center, Santa Monica, CA, USA
5Department of Pathology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA

Tóm tắt

AbstractThe MAGEA gene family that encodes cancer testis antigens is differentially expressed in many cancers. Though MAGEA3 expression has been detected in gastrointestinal malignancies, its role in pancreatic ductal adenocarcinoma (PDAC) has not been well established. We assessed 57 patients who underwent intent‐to‐cure surgery for PDAC. Total RNA from paraffin‐embedded pancreatic tumors was extracted and assessed for MAGEA3 gene expression by an optimized probe‐based quantitative real‐time RT‐PCR (qRT) assay. MAGEA3 gene expression was detected by qRT in 25 (44%) patients. For the entire cohort, detection of MAGEA3 expression was associated with significantly decreased overall survival (median, 16 vs 33 months; log‐rank, p = 0.032). When clinicopathologic factors, including age, gender, stage, tumor extent, lymph node metastasis, tumor grade, perineural invasion and lymphovascular invasion were assessed by univariate analysis, MAGEA3 gene expression and tumor grade were significant prognostic factors for poor survival (HR 2.1, 95% CI: 1.0–4.4, p = 0.041; and HR 3.7, 95% CI: 1.8–7.6, p = 0.0004, respectively). Immunohistochemistry (IHC) was performed and confirmed MAGEA3 protein in PDAC specimens. In conclusion, MAGEA3 is differentially expressed in patients with PDAC; its expression correlates with significantly worse survival. Molecular assessment for MAGEA3 should be considered to improve prognostic evaluation and to identify eligible patients for potential immune‐based therapy. © 2005 Wiley‐Liss, Inc.

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International Journal of Cancer

Tập 118 Số 9

2269-2275

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