The chelating peptide (GPAGPHGPPG) derived from Alaska pollock skin enhances calcium, zinc and iron transport in Caco‐2 cells

International Journal of Food Science and Technology - Tập 52 Số 5 - Trang 1283-1290 - 2017
Qianru Chen1, Lidong Guo1, Fen Du1, Tiejun Chen1, Hu Hou1, Bafang Li1
1College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong Province 266003, China

Tóm tắt

SummaryGly‐Pro‐Ala‐Gly‐Pro‐His‐Gly‐Pro‐Pro‐Gly (GPAGPHGPPG), a chelating peptide derived from Alaska pollock skin, has been approved with outstanding ability to chelate calcium, zinc and iron ions. In this study, the stability of GPAGPHGPPG during in vitro gastrointestinal enzymatic digestion, the potential binding site between peptide and metal ions as well as its effects on mineral transport in Caco‐2 cells were investigated. Results showed that approximately 75% of GPAGPHGPPG remained intact at the end of the in vitro gastrointestinal enzymatic digestion. The formation of peptide‐metal complex was potentially related to histidine and cyclic structure in terms of dehydration. Moreover, GPAGPHGPPG revealed significant promotional effects on calcium (112.7%, P < 0.01), zinc (32.3%, P < 0.01) and iron (27.7%, P < 0.01) transport in Caco‐2 cell monolayer. In conclusion, GPAGPHGPPG could be developed as a potential functional ingredient to prevent mineral deficiency.

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Thông tin
Thông tin xuất bản

International Journal of Food Science and Technology

Tập 52 Số 5

1283-1290

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