The RNA Component of Human Telomerase

American Association for the Advancement of Science (AAAS) - Tập 269 Số 5228 - Trang 1236-1241 - 1995
Junli Feng1, Walter D. Funk1, Sy-Shi Wang1, Scott L. Weinrich1, Ariel A. Avilion2, Choy‐Pik Chiu1, Robert Adams1, Edwin Chang1, Richard Allsopp1, Jinghua Yu1, Siyuan Le2, Michael D. West1, Calvin B. Harley1, William H. Andrews1, Carol W. Greider2, Bryant Villeponteau1
1Geron Corporation, Menlo Park, CA 94025, USA
2Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724 USA

Tóm tắt

Eukaryotic chromosomes are capped with repetitive telomere sequences that protect the ends from damage and rearrangements. Telomere repeats are synthesized by telomerase, a ribonucleic acid (RNA)-protein complex. Here, the cloning of the RNA component of human telomerase, termed hTR, is described. The template region of hTR encompasses 11 nucleotides (5′-CUAACCCUAAC) complementary to the human telomere sequence (TTAGGG) n . Germline tissues and tumor cell lines expressed more hTR than normal somatic cells and tissues, which have no detectable telomerase activity. Human cell lines that expressed hTR mutated in the template region generated the predicted mutant telomerase activity. HeLa cells transfected with an antisense hTR lost telomeric DNA and began to die after 23 to 26 doublings. Thus, human telomerase is a critical enzyme for the long-term proliferation of immortal tumor cells.

Từ khóa


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