The PsbZ subunit of Photosystem II in Synechocystis sp. PCC 6803 modulates electron flow through the photosynthetic electron transfer chain

Photosynthesis Research - Tập 93 - Trang 139-147 - 2007
Cleo L. Bishop1,2, Simin Ulas3, Elena Baena-Gonzalez3,4, Eva-Mari Aro3, Saul Purton1, Jonathan H. A. Nugent1, Pirkko Mäenpää3
1Photosynthesis Research Group, Department of Biology, University College London, London, UK
2Institute of Cellular and Molecular Sciences, Bart’s and the London Queen Mary’s School of Medicine and Dentistry, London, UK
3Department of Biology, Turku University, Turku, Finland
4Harvard Medical School, Boston, USA;

Tóm tắt

The psbZ gene of Synechocystis sp. PCC 6803 encodes the ∼6.6 kDa photosystem II (PSII) subunit. We here report biophysical, biochemical and in vivo characterization of Synechocystis sp. PCC 6803 mutants lacking psbZ. We show that these mutants are able to perform wild-type levels of light-harvesting, energy transfer, PSII oxygen evolution, state transitions and non-photochemical quenching (NPQ) under standard growth conditions. The mutants grow photoautotrophically; however, their growth rate is clearly retarded under low-light conditions and they are not capable of photomixotrophic growth. Further differences exist in the electron transfer properties between the mutants and wild type. In the absence of PsbZ, electron flow potentially increased through photosystem I (PSI) without a change in the maximum electron transfer capacity of PSII. Further, rereduction of P700+ is much faster, suggesting faster cyclic electron flow around PSI. This implies a role for PsbZ in the regulation of electron transfer, with implication for photoprotection.

Tài liệu tham khảo

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