The Occurrence of Mycoplasmas in the Lungs of Swine in Gran Canaria (Spain)

Veterinary Research Communications - Tập 29 - Trang 453-462 - 2005
P. Assunça˜o1, C. De la Fe1, B. Kokotovic2, O. González3, J. B. Poveda1
1Unidad de Epidemiología y Medicina Preventiva, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, Arucas, Spain
2Department of Veterinary Diagnostics and Research, Danish Institute for Food and Veterinary Research, Copenhagen V, Denmark
3Departamento de I+D Laboratorios Syva, Léon, Spain

Tóm tắt

The study was conducted to investigate the mycoplasmal flora in the lungs of pigs with enzootic pneumonia at Gran Canaria (Spain). From 54 pneumonic lungs collected at an abattoir, 85 isolates were cultivated. On the basis of cultural and biochemical characteristics, the isolates were preliminarily identified as Mycoplasma species. Using different species-specific PCRs, 40, 27, 11 and 7 of the isolates were identified as M. hyorhinis, M. hyopneumoniae, M. hyosynoviae and M. flocculare, respectively. Nine of the M. hyopneumoniae cultures were found to be in mixed culture with M. flocculare as demonstrated by PCR. By use of a M. flocculare antiserum it was possible to eliminate M. flocculare from M. hyopneumoniae mixed cultures. This study is the first report on isolation of porcine mycoplasmas at Gran Canaria (Spain).

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Veterinary Research Communications

Tập 29

453-462

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