The Legionella micdadei flagellin: Expression in Escherichia coli K 12 and DNA sequence of the gene

APMIS - Tập 103 Số 7-8 - Trang 869-877 - 1995
Jette Bangsborg1,2, Peter Hindersson2, G H Shand1, Niels Høiby1,2
1Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark
2Institute of Medical Microbiology and Immunology, University of Copenhagen

Tóm tắt

To study the structure and function of the Legionella flagellum, we screened a genomic L. micdadei library in Escherichia coli for expression of the flagellin (Fla) subunit. One recombinant clone, JM105 (pHI5588), producing a truncated Fla protein of 40.5 kDa was identified. The plasmid pHI5588 carried a L. micdadei DNA insert of 5 kb, containing ca 95% of the fla gene. The complete DNA sequence of the L. micdadei fla gene was obtained by combining sequence data from pHI5588 with results using a polymerase chain reaction‐based system for genome walking (vectoretle PCR). The L. micdadei fla gene shared a high degree of homology with other flagellin genes in the amino‐ and carboxy termini, whereas the central region was found to be nonconserved. The fla sequence will facilitate the cloning of Fla proteins from other Legionella species and the study of flagella in the pathogenesis of Legionnaires' disease.

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APMIS

Tập 103 Số 7-8

869-877

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