The Impact of Different Long‐Term Storage Conditions on the Viability of Lichen‐Forming Ascomycetes and their Green Algal Photobiont, Trebouxia spp.

Plant Biology - Tập 5 Số 3 - Trang 324-330 - 2003
Rosmarie Honegger1
1Institute of Plant Biology, University of Zürich, Zürich, Switzerland

Tóm tắt

Abstract: Lichen‐forming ascomycetes and their green algal photobionts completely die off within approximately 3 years of storage at room temperature. Macroscopically this is recognizable as a colour change, the green shades of the chlorophylls being lost. In fluorescent light microscopy preparations an increase in fungal autofluorescence and a significant decrease in chlorophyll autofluorescence in the Trebouxia cells was observed. In transmission electron microscopy preparations of Xanthoria parietina and its green algal photobiont, Trebouxia arboricola, the fungal membrane systems were found to be largely broken down whereas the shrivelled algal protoplast failed to rehydrate after storage at room temperature. When stored in the desiccated state at ‐ 20 °C, both partners of the symbiosis stayed fully viable for up to 13 years, their colouration and chlorophyll fluorescence being unchanged. Viability was measured as ascospore ejection and germination rates in Xanthoria parietina, soredium germination rates in Xanthoria fallax, Hypogymnia physodes and Parmelia sulcata, and autospore formation rate in Trebouxia cells (green algal photobiont), which had been isolated from the thalli after rehydration. Thallus fragments of Xanthoria parietina were shown to grow normally after one week of storage in LN2 without any cryoprotectant. In the desiccated state deep‐frozen samples can be repeatedly brought to room temperature and back to ‐ 20 °C without any loss of viability. Cryopreservation is therefore a suitable mode of long‐term storage of viable lichen thalli for experimental studies or transplant experiments.

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Tài liệu tham khảo

Becquerel P., 1948, Réviviscence du Xanthoria parietina desseché avec sa faune, six ans dans le vide et deux semaines à ‐ 189 °C. Ses consequences biologiques, Comptes rendus de l'Académie des Sciences, 226, 1413

10.1094/Phyto-73-241

10.1007/BF00936349

10.1111/j.1529-8817.1995.tb02559.x

Deason T. R., 1960, Phycological studies. Exploratory studies of Texas soil algae

Hohl H. R., 1987, Zoosporic Fungi in Teaching and Research, 143

Honegger R., 1993, A simple outdoor culturing technique for the foliose macrolichens Xanthoria parietina and Parmelia sulcata., Botanica Helvetica, 103, 223

10.1139/b95-297

Honegger R., 1996, The Biology of Lichens, 25

10.1007/BF01281320

10.1017/S002428299200015X

Kappen L., 1988, CRC Handbook of Lichenology, 37

Keller P., 1995, Resistance: are there limits to resistance in lichens, Symbiosis, 18, 87

Lange O. L., 1953, Hitze‐ und Trockenresistenz der Flechten in Beziehung zu ihrer Verbreitung, Flora, 140, 39

Marti J., 1985, Die Toxizität von Zink, Schwefel‐ und Stickstoffverbindungen auf Flechten‐Symbionten, Bibliotheca Lichenologica, 21, 129

Wyndham J., 1960, Trouble with Lichen

Yamamoto Y., 1998, Factors affecting discharge and germination of lichen ascospores, Journal of the Hattori Botanical Laboratory, 85, 267

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Plant Biology

Tập 5 Số 3

324-330

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