The Fluorescent Toolbox for Assessing Protein Location and Function

American Association for the Advancement of Science (AAAS) - Tập 312 Số 5771 - Trang 217-224 - 2006
Ben N. G. Giepmans1,2,3, Stephen Adams1,2,3, Mark H. Ellisman1,2,3, Roger Y. Tsien1,2,3
1Department of Chemistry and Biochemistry and Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093, USA.
2Department of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA
3National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA

Tóm tắt

Advances in molecular biology, organic chemistry, and materials science have recently created several new classes of fluorescent probes for imaging in cell biology. Here we review the characteristic benefits and limitations of fluorescent probes to study proteins. The focus is on protein detection in live versus fixed cells: determination of protein expression, localization, activity state, and the possibility for combination of fluorescent light microscopy with electron microscopy. Small organic fluorescent dyes, nanocrystals (“quantum dots”), autofluorescent proteins, small genetic encoded tags that can be complexed with fluorochromes, and combinations of these probes are highlighted.

Từ khóa


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We apologize that many original research papers could not be cited because of space limitations. Work by the authors directly related to this review is supported by NIH RR04050 NS27177 GM 72033 and by the Howard Hughes Medical Institute.