The Effect of Leonurus sibiricus Plant Extracts on Stimulating Repair and Protective Activity against Oxidative DNA Damage in CHO Cells and Content of Phenolic Compounds

Oxidative Medicine and Cellular Longevity - Tập 2016 Số 1 - 2016
Przemysław Sitarek1, Ewa Skała1, Halina Wysokińska1, Marzena Wielanek2, Janusz Szemraj3, Monika M. Toma4, Tomasz Śliwiński4
1Department of Biology and Pharmaceutical Botany, Medical University of Łódź, Muszyńskiego 1, 90-151 Łódź, Poland
2Department of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland
3Department of Medical Biochemistry, Medical University of Łódź, Mazowiecka 6/8, 92-215 Łódź, Poland.
4Department of Molecular Genetics, University of Łódź, Pomorska 141/143, 90-236, Łódź, Poland

Tóm tắt

Leonurus sibiricus L. has been used as a traditional and medicinal herb for many years in Asia and Europe. This species is known to have antibacterial, anti‐inflammatory, and antioxidant activity and has demonstrated a reduction of intracellular reactive oxygen species. All tested extracts of L. sibiricus showed protective and DNA repair stimulating effects in Chinese hamster ovary (CHO) cells exposed to H2O2. Preincubation of the CHO cells with 0.5 mg/mL of plant extracts showed increased expression level of antioxidant genes (SOD2, CAT, and GPx). LC‐MS/MS and HPLC analyses revealed the presence of nine phenolic compounds in L. sibiricus plant extracts: catechin, verbascoside, two flavonoids (quercetin and rutin), and five phenolic acids (4‐hydroxybenzoic acid, chlorogenic acid, caffeic acid, p‐coumaric acid, and ferulic acid). The roots and aerial parts of in vitro L. sibiricus plant extracts, which had the strongest antioxidant properties, may be responsible for stimulating CHO cells to repair oxidatively induced DNA damage, as well as protecting DNA via enhanced activation of the antioxidant genes (SOD2, CAT, and GPx) regulating intracellular antioxidant capacity. The content of phenolic compounds in in vitro raised plants was greater than the levels found in plants propagated from seeds.

Từ khóa


Tài liệu tham khảo

10.1021/np100956k

10.1007/s11418-013-0781-0

10.1016/j.jep.2013.08.013

10.1016/j.fitote.2005.03.014

10.1016/j.fitote.2006.03.005

10.1080/08923970802135443

10.1271/bbb.90582

10.1016/j.pathophys.2006.05.002

10.1016/j.jep.2013.12.052

10.1111/j.1399-3054.1962.tb08052.x

10.1016/0022-1759(83)90303-4

10.1016/j.mrfmmm.2007.08.017

10.1385/mb:26:3:249

Sheng-Ming P., 2006, Phenols from the aerial parts of Leonurus sibiricus, The Chinese Pharmacutical Journal, 58, 35

10.1111/j.1749-6632.1998.tb09922.x

10.1021/jf990146l

10.1080/10715760210161

10.1079/bjn2003849

10.1002/mnfr.200500136

Chua H. P., 2013, Determination of antioxidant activities of dried kacangma (Leonurus sibiricus) extract in three bioassay systems, Journal of Tropical Agriculture and Food Science, 41, 221

10.1096/fj.02-0752rev

10.1093/molbev/msm276

10.1080/15216540410001701642

10.1186/s12950-015-0055-6

10.1371/journal.pone.0107670

10.1152/ajpendo.90921.2008

Thông tin
Thông tin xuất bản

Oxidative Medicine and Cellular Longevity

Tập 2016 Số 1

Thông tin tác giả