The Effect of Liu-Wei-Di-Huang Wan on Cytokine Gene Expression from Human Peripheral Blood Lymphocytes

American Journal of Chinese Medicine - Tập 31 Số 02 - Trang 247-257 - 2003
Jiann‐Jong Shen1, Chunjing Lin2, Jing‐Long Huang3, Kue‐Hsiung Hsieh3, Ming‐Ling Kuo2,4
1Department of Chinese Medicine, Chang Gung University, Taoyuan, Taiwan, Republic of China.
2Department of Microbiology and Immunology, Chang Gung University, Taoyuan, Taiwan Republic of China
3Division of Allergy, Asthma, and Rheumatology, Chang Gung Children's Hospital, Taoyuan, Taiwan, Republic of China
4Medical Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan (Republic of China)

Tóm tắt

Liu-Wei-Di-Huang Wan (LWDHW) has been used by traditional Chinese doctors to treat asthma patients. This study was to examine the potential effect of this decoction on the regulation of T helper (Th)1- and Th2-type cytokine gene expression in vitro. Peripheral blood mononuclear cells (PBMC) were activated with mitogen for 24 hours in the presence or absence of LWDHW extracts. Concentrations of different cytokines in the culture supernatants were determined with ELISA. RNA isolated from cultured cells was subjected to RT-PCR analysis. The results showed that the expression of all cytokines (Th2-type: IL-4, IL-5, IL-10, or IL-13 and Th1-type: IL-2 and IFN-γ) examined was inhibited at both RNA and protein levels by LWDHW. Since the cell viability was similar in all cultures, the reduction of cytokine production was not due to the toxicity of LWDHW. Moreover, the cells either retained or increased their capacity to respond to mitogen stimulation after incubation with the LWDHW decoction. Therefore, the data suggest that LWDHW functioned directly on cytokine gene expression from activated PBMC.

Từ khóa


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Thông tin xuất bản

American Journal of Chinese Medicine

Tập 31 Số 02

247-257

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