The 1.9 Å crystal structure of Escherichia coli MurG, a membrane‐associated glycosyltransferase involved in peptidoglycan biosynthesis

Protein Science - Tập 9 Số 6 - Trang 1045-1052 - 2000
Sha Ha1, David Walker, Yigong Shi, Suzanne Walker
1Department of Chemistry, Princeton University, New Jersey 08544, USA

Tóm tắt

AbstractThe 1.9 Å X‐ray structure of a membrane‐associated glycosyltransferase involved in peptidoglycan biosynthesis is reported. This enzyme, MurG, contains two α/β open sheet domains separated by a deep cleft. Structural analysis suggests that the C‐terminal domain contains the UDP‐GlcNAc binding site while the N‐terminal domain contains the acceptor binding site and likely membrane association site. Combined with sequence data from other MurG homologs, this structure provides insight into the residues that are important in substrate binding and catalysis. We have also noted that a conserved region found in many UDP‐sugar transferases maps to a β/α/β/α supersecondary structural motif in the donor binding region of MurG, an observation that may be helpful in glycosyltransferase structure prediction. The identification of a conserved structural motif involved in donor binding in different UDP‐sugar transferases also suggests that it may be possible to identify—and perhaps alter—the residues that help determine donor specificity.

Từ khóa


Tài liệu tham khảo

10.1016/0022-2836(92)90848-E

10.1038/nsb0895-644

10.1093/emboj/16.12.3416

Branden C, 1998, Introduction to protein structure

10.1107/S0907444998003254

BuggTDH WalshCT.1992.Intracellular steps of bacterial cell wall peptidoglycan biosynthesis: Enzymology antibiotics and antibiotic resistance.Nat Prod Rep199–215.

10.1128/jb.175.6.1841-1843.1993

10.1002/(SICI)1097-0134(199705)28:1<10::AID-PROT2>3.0.CO;2-N

CCP4 (Collaborative Computing Project Number 4), 1994, The CCP4 suite: Programs for protein crystallography, Acta Crystallogr D, 50, 760, 10.1107/S0907444994003112

10.1021/bi990270y

10.1016/S0304-4165(99)00167-1

10.1126/science.7939684

10.1093/emboj/18.13.3546

10.1021/ja991556t

10.1107/S0108767390010224

10.1093/glycob/9.10.961

10.1096/fasebj.13.15.2329

10.1107/S0021889891004399

Mackenzie PI, 1990, Frontiers in biotransformation: Principles, mechanisms and biological consequences of induction, 211, 10.1515/9783112563304-009

10.1021/ja974221p

10.1107/S0907444994006396

10.1006/jmbi.1999.3094

10.1016/S0076-6879(97)76066-X

10.1126/science.287.5460.2007

10.1016/S0969-2126(96)00153-0

10.1016/S0304-4165(99)00174-9

10.1002/j.1460-2075.1994.tb06646.x

Thông tin
Thông tin xuất bản

Protein Science

Tập 9 Số 6

1045-1052

Thông tin tác giả