Textured soy protein scaffolds enable the generation of three-dimensional bovine skeletal muscle tissue for cell-based meat

Nature Food - Tập 1 Số 4 - Trang 210-220
Tom Ben‐Arye1, Yulia Shandalov1, Shahar Ben‐Shaul1, Shira Landau1, Yedidya Zagury1, Iris Ianovici1, Neta Lavon2, Shulamit Levenberg3
1Department of Biomedical Engineering, Technion–Israel Institute of Technology, Haifa, Israel
2Aleph Farms Ltd, Ashdod, Israel
3Russell Berrie Nanotechnology Institute, Technion, Israel Institute of Technology, Haifa, Israel

Tóm tắt

Từ khóa


Tài liệu tham khảo

Post, M. & Weele, C. Principles of Tissue Engineering for Food (Elsevier, 2014).

Slade, P. If you build it, will they eat it? Consumer preferences for plant-based and cultured meat burgers. Appetite 125, 428–437 (2018).

Ben-Arye, T. & Levenberg, S. Tissue engineering for clean meat production. Front. Sustain. Food Syst. 3, 46 (2019).

Specht, E. A., Welch, D. R., Rees Clayton, E. M. & Lagally, C. D. Opportunities for applying biomedical production and manufacturing methods to the development of the clean meat industry. Biochem. Eng. J. 132, 161–168 (2018).

Edelman, P. D., McFarland, D. C., Mironov, V. A. & Matheny, J. G. Commentary: in vitro-cultured meat production. Tissue Eng. 11, 659–662 (2005).

Egozi, D. et al. Engineered vascularized muscle flap. J. Vis. Exp. 107, 52984 (2016).

Gholobova, D. et al. Endothelial network formation within human tissue-engineered skeletal muscle. Tissue Eng. Part A 21, 2548–2558 (2015).

Levenberg, S. Engineering blood vessels from stem cells: recent advances and applications. Curr. Opin. Biotechnol. 16, 516–523 (2005).

Shandalov, Y. et al. An engineered muscle flap for reconstruction of large soft tissue defects. Proc. Natl Acad. Sci. USA 111, 6010–6015 (2014).

Listrat, A. et al. How muscle structure and composition influence meat and flesh quality. Sci. World J. 2016, 3182746 (2016).

Vitello, L. et al. Enhancing myoblast proliferation by using myogenic factors: a promising approach for improving fiber regeneration in sport medicine and skeletal muscle diseases. Basic Appl. Myol. 14, 45–51 (2004).

Yin, H., Price, F. & Rudnicki, M. A. Satellite cells and the muscle stem cell niche. Physiol. Rev. 93, 23–67 (2013).

Purslow, P. P. Muscle fascia and force transmission. J. Bodyw. Mov. Ther. 14, 411–417 (2010).

Jockenhoevel, S. et al. Fibrin gel—advantages of a new scaffold in cardiovascular tissue engineering. Eur. J. Cardiothorac. Surg. 19, 424–430 (2001).

Guo, B. et al. Transcriptome analysis of cattle muscle identifies potential markers for skeletal muscle growth rate and major cell types. BMC Genomics 16, 177 (2015).

Jain, R. K., Au, P., Tam, J., Duda, D. G. & Fukumura, D. Engineering vascularized tissue. Nat. Biotechnol. 23, 821–823 (2005).

Christov, C. et al. Muscle satellite cells and endothelial cells: close neighbors and privileged partners. Mol. Biol. Cell 18, 1397–1409 (2007).

Butler, J. M., Kobayashi, H. & Rafii, S. Instructive role of the vascular niche in promoting tumour growth and tissue repair by angiocrine factors. Nat. Rev. Cancer 10, 138–146 (2010).

Rafii, S., Butler, J. M. & Ding, B.-S. Angiocrine functions of organ-specific endothelial cells. Nature 529, 316–325 (2016).

Kyriakopoulou, K., Dekkers, B. & van der Goot, A. J. in Sustainable Meat Production and Processing (ed. Galanakis, C. M.) 103–126 (Academic Press, 2019).

Day, L. Proteins from land plants—potential resources for human nutrition and food security. Trends Food Sci. Technol. 32, 25–42 (2013).

Zeltinger, J., Sherwood, J. K., Graham, D. A., Müeller, R. & Griffith, L. G. Effect of pore size and void fraction on cellular adhesion, proliferation, and matrix deposition. Tissue Eng. 7, 557–572 (2001).

Hayes, J. S., Czekanska, E. M. & Richards, R. G. in Tissue Engineering III: Cell-Surface Interactions for Tissue Culture (eds Kasper, C., Witte, F. & Pörtner, R.) 1–31 (Springer, 2012).

Rodriguez, B. L. & Larkin, L. M. in Functional 3D Tissue Engineering Scaffolds (eds Deng, Y. & Kuiper, J.) 279–304 (Woodhead Publishing, 2018).

Choi, J. S., Lee, S. J., Christ, G. J., Atala, A. & Yoo, J. J. The influence of electrospun aligned poly(epsilon-caprolactone)/collagen nanofiber meshes on the formation of self-aligned skeletal muscle myotubes. Biomaterials 29, 2899–2906 (2008).

Aviss, K. J., Gough, J. E. & Downes, S. Aligned electrospun polymer fibres for skeletal muscle regeneration. Eur. Cells Mater. 19, 193–204 (2010).

Levenberg, S. et al. Engineering vascularized skeletal muscle tissue. Nat. Biotechnol. 23, 879–884 (2005).

Perry, L., Landau, S., Flugelman, M. Y. & Levenberg, S. Genetically engineered human muscle transplant enhances murine host neovascularization and myogenesis. Commun. Biol. 1, 161 (2018).

Specht, L. An Analysis of Culture Medium Costs and Production Volumes for Cell-Based Meat (The Good Food Institute, 2019).

Loh, Q. L. & Choong, C. Three-dimensional scaffolds for tissue engineering applications: role of porosity and pore size. Tissue Eng. Part B Rev. 19, 485–502 (2013).

Du, M., Wang, B., Fu, X., Yang, Q. & Zhu, M.-J. Fetal programming in meat production. Meat Sci. 109, 40–47 (2015).

Ding, S. et al. Maintaining bovine satellite cells stemness through p38 pathway. Sci. Rep. 8, 10808 (2018).

Verbruggen, S., Luining, D., van Essen, A. & Post, M. J. Bovine myoblast cell production in a microcarriers-based system. Cytotechnology 70, 503–512 (2018).

Péault, B. et al. Stem and progenitor cells in skeletal muscle development, maintenance, and therapy. Mol. Ther. 15, 867–877 (2007).

Du, M., Huang, Y., Das, A. K., Yang, Q. & Duarte, M. S. Manipulating mesenchymal progenitor cell differentiation to optimize performance and carcass value of beef cattle. J. Anim. Sci. 91, 1419–1427 (2013).

Chapman, M. A., Meza, R. & Lieber, R. L. Skeletal muscle fibroblasts in health and disease. Differentiation 92, 108–115 (2016).

Krieger, J., Park, B.-W., Lambert, C. R. & Malcuit, C. 3D skeletal muscle fascicle engineering is improved with TGF-β1 treatment of myogenic cells and their co-culture with myofibroblasts. PeerJ. 6, e4939 (2018).

Bauman, T. M. et al. Characterization of fibrillar collagens and extracellular matrix of glandular benign prostatic hyperplasia nodules. PLoS ONE 9, e109102 (2014).

Suvik, A. & Effendy, A. W. M. The use of modified Masson’s trichrome staining in collagen evaluation in wound healing study. Mal. J. Vet. Res. 3, 39–47 (2012).

Mehta, F., Theunissen, R. & Post, M. J. in Myogenesis: Methods and Protocols (ed. Rønning, S. B.) 111–125 (Springer, 2019).

Frey, R. S., Johnson, B. J., Hathaway, M. R., White, M. E. & Dayton, W. R. Growth factor responsiveness of primary satellite cell cultures from steers implanted with trenbolone acetate and estradiol-17β. Basic Appl. Myol. 5, 71–79 (1995).

Lapin, M. R., Gonzalez, J. M. & Johnson, S. E. Substrate elasticity affects bovine satellite cell activation kinetics in vitro. J. Anim. Sci. 91, 2083–2090 (2013).

Lu, R., Chen, Y.-R., Solomon, M. B. & Berry, B. W. Tensile properties and Warner–Bratzler tenderness measurement of raw and cooked beef. Trans. ASAE 41, 1431–1439 (1998).