Temporality in Ultrasound‐Induced Cell Lysis In Vitro

Echocardiography - Tập 13 Số 1 - Trang 45-55 - 1996
Mitra Azadniv1, Yukio Doida2, Morton W. Miller1, Andrew A. Brayman1, Richard S. Meltzer3
1Department of Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York
2Department of Medicine, Cardiology Unit, School of Medicine and Dentistry, University of Rochester, Rochester, New York
3Department of Biology, Shiga University of Medical Science, Shiga, Japan

Tóm tắt

To test the hypothesis that the full extent of in vitro cell lysis due to ultrasound becomes evident with time lapse following insonation, human erythrocytes (2% hematocrit) in autologous plasma were mixed with Albunex®, a pulse echo contrast agent, and exposed to 1‐MHz, continuous‐wave ultrasound (US) (5 W/cm2 SPTA intensity) for 60 seconds while in a rotating (200 rpm) dialysis membrane vessel. Exposed and sham‐exposed samples were subsequently assayed for hemolysis colorimetrically, either immediately or after a delay of 3 hours. Hemolysis was dependent on the interval between US exposure and assay, with significantly greater lysis evident with delayed assay. There was also temporality in lytic yield with sample number, i.e., with time postpreparation of the blood sample, US‐induced cell lysis decreased. The temporality of lytic yield was eliminated by maintenance at ice water temperatures, or by waiting about 1 hour before beginning treatments. The collective data indicate that the full extent of US‐induced cell lysis is not evident upon assay immediately after insonation, and that with time postpreparation and preinsonation, erythrocytes may undergo changes in sensitivity to US.

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Tài liệu tham khảo

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Thông tin xuất bản

Echocardiography

Tập 13 Số 1

45-55

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