Targeted Multiplex Imaging Mass Spectrometry in Transmission Geometry for Subcellular Spatial Resolution
Tóm tắt
Targeted multiplex imaging mass spectrometry utilizes several different antigen-specific primary antibodies, each directly labeled with a unique photocleavable mass tag, to detect multiple antigens in a single tissue section. Each photocleavable mass tag bound to an antibody has a unique molecular weight and can be readily ionized by laser desorption ionization mass spectrometry. This article describes a mass spectrometry method that allows imaging of targeted single cells within tissue using transmission geometry laser desorption ionization mass spectrometry. Transmission geometry focuses the laser beam on the back side of the tissue placed on a glass slide, providing a 2 μm diameter laser spot irradiating the biological specimen. This matrix-free method enables simultaneous localization at the sub-cellular level of multiple antigens using specific tagged antibodies. We have used this technology to visualize the co-expression of synaptophysin and two major hormones peptides, insulin and somatostatin, in duplex assays in beta and delta cells contained in a human pancreatic islet.
Tài liệu tham khảo
Caprioli, R.M., Farmer, T.B., Gile, J.: Molecular imaging of biological samples: localization of peptides and proteins using MALDI-TOF MS. Anal. Chem. 69(23), 4751–4760 (1997)
Thiery, G., Shchepinov, M.S., Southern, E.M., Audebourg, A., Audard, V., Terris, B., Gut, I.G.: Multiplex target protein imaging in tissue sections by mass spectrometry - TAMSIM. Rapid Commun. Mass Spectrom. 21(6), 823–829 (2007)
Thiery, G., Anselmi, E., Audebourg, A., Darii, E., Abarbri, M., Terris, B., Tabet, J.C., Gut, I.G.: Improvements of targeted multiplex mass spectrometry imaging. Proteomics 8(18), 3725–3734 (2008)
Bian, N., Wang, P., Wang, Z., Xu, L., Church, G., Giese, R.W.: Detection via laser desorption and mass spectrometry of multiplex electrophore-labeled albumin. Rapid Commun. Mass Spectrom. 11(16), 1781–1784 (1997)
Galicia, M.C., Vertes, A., Callahan, J.H.: Atmospheric pressure matrix-assisted laser desorption/ionization in transmission geometry. Anal. Chem. 4(8), 1891–1895 (2002)
Richards, A.L., Lietz, C.B., Wager-Miller, J.B., Mackie, K., Trimpin, S.: Imaging mass spectrometry in transmission geometry. Rapid Commun. Mass Spectrom. 25(6), 815–820 (2011)
Schurenberg, M., Schulz, T.D.: K., Hillenkamp, F.: Matrix-assisted laser desorption/ionization in transmission geometry: instrumental implementation and mechanistic implications. Rapid Commun. Mass Spectrom. 10(15), 1873–1880 (1996)
Vaeck, L.V., Struyf, H., Roy, W.V., Adams, F.: Organic and inorganic analysis with laser microprobe mass spectrometry. Part I: Instrumentation and methodology. Mass Spectrom. Rev. 13(3), 189–208 (1994)
Vertes, A., Balazs, L., Gijbels, R.: Matrix-assisted Laser desorption of peptides in transmission geometry. Rapid Commun. Mass Spectrom. 4(7), 263–266 (1990)
Vogt, H., Heinen, H.J., Meier, S., Wechsung, R.: LAMMA 500 principle and technical description of the instrument. Fresenius Z J. Anal. Chem. 308(3), 195–200 (1981)
Zavalin, A.I., Yang, J., Caprioli, R.M.: Tissue imaging at submicron spatial resolution using direct transmission geometry MALDI-MS. Proceedings of the 60th ASMS Conference on Mass Spectrometry and Allied Topic, Vancouver, BC (2012)
Zavalin, A., Todd, E., Rawhouser, P., Yang, J., Norris, J., Caprioli, R.: Direct imaging of single cells and tissue at subcellular spatial resolution using transmission geometry MALDI-MS. J. Mass Spectrom. 47(11), 1473–1481 (2012)
Dannies, P.S.: Protein hormone storage in secretory granules: mechanisms for concentration and sorting. Endocr. Rev. 20(1), 3–21 (1999)
Portela-Gomes, G.M., Lukinius, A., Grimelius, L.: Synaptic vesicle protein 2, a new neuroendocrine cell marker. Am. J. Pathol. 157(4), 1299–1309 (2000)
Moriyama, Y., Hayashi, M., Yamada, H., Yatsushiro, S., Ishio, S., Yamamoto, A.: Synaptic-like microvesicles, synaptic vesicle counterparts in endocrine cells, are involved in a novel regulatory mechanism for the synthesis and secretion of hormones. J. Exp. Biol. 203(Pt 1), 117–125 (2000)
Reetz, A., Solimena, M., Matteoli, M., Folli, F., Takei, K., De Camilli, P.: GABA and pancreatic beta-cells: colocalization of glutamic acid decarboxylase (GAD) and GABA with synaptic-like microvesicles suggests their role in GABA storage and secretion. EMBO J. 10(5), 1275–1284 (1991)
Lukinius, A., Stridsberg, M., Wilander, E.: Cellular expression and specific intragranular localization of chromogranin A, chromogranin B, and synaptophysin during ontogeny of pancreatic islet cells: an ultrastructural study. Pancreas 27(1), 38–46 (2003)
Portela-Gomes, G.M., Gayen, J.R., Grimelius, L., Stridsberg, M., Mahata, S.K.: The importance of chromogranin A in the development and function of endocrine pancreas. Regulatory Peptides 2008(151), 19–25 (2008)
Portela-Gomes, G.M., Hacker, G.W., Weitgasser, R.: Neuroendocrine cell markers for pancreatic islets and tumors. Appl. Immunohistochem. Mol. Morphol. 12(3), 183–192 (2004)
Thiery, G., Mernaugh, R. L., Yan, H., Spraggins, J. M., Yang, J., Parl, F. F., Caprioli, R. M.: Targeted multiplex imaging mass spectrometry with single chain fragment variable (SCFV) recombinant antibodies. J. Am. Soc. Mass Spectrom. 23, 1689–1696 (2012)
Shchepinov, M.S., Chalk, R., Southern, E.M.: Trityl mass-tags for encoding in combinatorial oligonucleotide synthesis. Nucleic Acids Symp. Ser. 42, 107–108 (1999)
Thiery, G., Zavalin, A.I., Caprioli, R.M.: Targeted multiplex mass spectrometry imaging in transmission geometry LDI for a sub-cellular resolution. Proceedings of the 58th Annual Conference on Mass Spectrometry, Salt Lake City, Utah (2010)
Leclerc, R., Pelletier, G., Puviani, R., Arimura, A., Schally, A.V.: Immunohistochemical localization of somatostatin in endocrine cells of the rat stomach. Mol. Cell. Endocrinol. 4(4), 257–261 (1976)
Portela-Gomes, G.M., Stridsberg, M., Johansson, H., Grimelius, L.: Co-localization of synaptophysin with different neuroendocrine hormones in the human gastrointestinal tract. Histochem. Cell. Biol. 111(1), 49–54 (1999)
Redecker, P., Jorns, A., Jahn, R., Grube, D.: Synaptophysin immunoreactivity in the mammalian endocrine pancreas. Cell Tissue Res. 264(3), 461–7 (1991)