TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis

Reproductive Biology and Endocrinology - 2010
Amelie Fassbender1, Péter Stella1, Cleophas Kyama1, Etienne Waelkens2, Attila Mihályi1, Christel Meuleman1, Olivier Gevaert3, Raf Van de Plas3, Bart De Moor3, Thomas D’Hooghe4
1Department of Obstetrics & Gynaecology, Leuven University Fertility Centre,University Hospital Gasthuisberg, Leuven, Belgium
2Biochemistry Section, Department of Molecular Cell Biology, Campus Gasthuisberg, Leuven, Belgium
3Department of Electrical Engineering, ESAT-SCD, K.U.Leuven, Kasteelpark-Arenberg 10, B-3001, Heverlee, Belgium
4Division of Reproductive Health and Biology, Institute of Primate Research, Karen, P.O. Box 24481-00502, Nairobi, Kenya

Tóm tắt

Abstract Background According to mRNA microarray, proteomics and other studies, biological abnormalities of eutopic endometrium (EM) are involved in the pathogenesis of endometriosis, but the relationship between mRNA and protein expression in EM is not clear. We tested for the first time the hypothesis that EM TRIzol extraction allows proteomic Surface Enhanced Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry (SELDI-TOF MS) analysis and that these proteomic data can be related to mRNA (microarray) data obtained from the same EM sample from women with and without endometriosis. Methods Proteomic analysis was performed using SELDI-TOF-MS of TRIzol-extracted EM obtained during secretory phase from patients without endometriosis (n = 6), patients with minimal-mild (n = 5) and with moderate-severe endometriosis (n = 5), classified according to the system of the American Society of Reproductive Medicine. Proteomic data were compared to mRNA microarray data obtained from the same EM samples. Results In our SELDI-TOF MS study 32 peaks were differentially expressed in endometrium of all women with endometriosis (stages I-IV) compared with all controls during the secretory phase. Comparison of proteomic results with those from microarray revealed no corresponding genes/proteins. Conclusion TRIzol treatment of secretory phase EM allows combined proteomic and mRNA microarray analysis of the same sample, but comparison between proteomic and microarray data was not evident, probably due to post-translational modifications.

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Reproductive Biology and Endocrinology

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