Tác động của việc chiết xuất hỗ trợ enzyme đến năng suất, thành phần và hoạt tính kháng khuẩn của tinh dầu từ cây Hương thảo (Rosmarinus officinalis L.) được trồng tại tỉnh Lâm Đồng, Việt Nam

CTU Journal of Innovation and Sustainable Development - Tập 14 Số 3 - Trang 65-71 - 2022
Van Ngoc Nguyen1, Tran Tham Thuy Do2, Bao Ngoc Vu2, Thi Binh Hoang2
1a:1:{s:5:"en_US";s:16:"Dalat University";}

Tóm tắt

Nghiên cứu này tiến hành tiền xử lý bằng enzyme viscozyme L đối với phần trên không của cây Hương thảo (Rosmarinus officinalis L.) được trồng tại tỉnh Lâm Đồng, nhằm đánh giá hiệu quả chiết xuất tinh dầu bằng phương pháp chưng cất thủy phân. Tiền xử lý enzym với 1 g enzyme viscozyme L trộn với 5 mL muối sodium chloride 15% ở nhiệt độ 50oC trong 1 giờ, tiếp theo là chưng cất thủy phân, cho thấy sự gia tăng nhẹ về năng suất tinh dầu Hương thảo từ 0.96% lên 1.08%. Phân tích GC/MS cho thấy có mặt 32 hợp chất trong tinh dầu Hương thảo, trong đó các thành phần chính là α-pinene (29.71 - 32.17%) và cineol (17.55 - 18.74%) ở cả mẫu kiểm soát và mẫu đã qua xử lý enzym. Kết quả cũng chỉ ra rằng tinh dầu Hương thảo thu được từ cả mẫu kiểm soát và mẫu đã qua xử lý enzym đều thể hiện hoạt tính kháng khuẩn ở mức độ trung bình đối với các vi khuẩn Escherichia coli, Staphylococcus aureus, và nấm Candida albicans ở tất cả các nồng độ thử nghiệm.

Từ khóa

#Antimicrobial activity #essential oil #enzyme assisted extraction #Lam Dong #Rosmarinus officinalis

Tài liệu tham khảo

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