Synthetic Gene Circuits: Design with Directed Evolution

Annual Reviews - Tập 36 Số 1 - Trang 1-19 - 2007
Eric L. Haseltine1, Frances H. Arnold2
1Division of Chemistry and Chemical Engineering, 210-41 California Institute of Technology, Pasadena, California 91125, USA.
2Division of Chemistry and Chemical Engineering 210-41, California Institute of Technology, Pasadena, California 91125;

Tóm tắt

Synthetic circuits offer great promise for generating insights into nature's underlying design principles or forward engineering novel biotechnology applications. However, construction of these circuits is not straightforward. Synthetic circuits generally consist of components optimized to function in their natural context, not in the context of the synthetic circuit. Combining mathematical modeling with directed evolution offers one promising means for addressing this problem. Modeling identifies mutational targets and limits the evolutionary search space for directed evolution, which alters circuit performance without the need for detailed biophysical information. This review examines strategies for integrating modeling and directed evolution and discusses the utility and limitations of available methods.

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Annual Reviews

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