Symptomatic cytomegalovirus (CMV) infections identified by image cytometry and other parameters for CMV infection

Transplant International - Tập 3 - Trang 212-216 - 1990
W. T. van Dorp1, E. Jonges1, N. M. Jiwa2, G. W. van Gemert3, L. A. van Es1, J. S. Ploem2, T. H. The4, F. J. van der Woude1
1Department of Nephrology, University Hospital Leiden, Leiden, The Netherlands
2Department of Cytochemistry and Cytometry, University Hospital Leiden, Leiden, The Netherlands
3Department of Virology, University Hospital Leiden, Leiden, The Netherlands
4Department of Clinical Immunology, Groningen University, Groningen, The Netherlands

Tóm tắt

Thirty-eight renal transplant recipients were followed during the first 3 months after transplantation. Once weekly, cultures of urine and buffy coat for cytomegalovirus (CMV) were taken and an immunocytochemical assay for immediate early antigens of CMV (IEA assay) was performed. Thirty patients had evidence of a CMV infection and 11 had a symptomatic CMV infection. All symptomatic patients had one or more positive urine cultures or a positive IEA assay. However, 15 patients with positive urine cultures and 12 patients with a positive IEA assay lacked any signs of symptomatic CMV disease. Moreover, 6 out of 15 patients with positive buffy coat cultures for CMV did not have symptomatic CMV disease. Using a computerized system to quantify IEA-positive granulocytes, we show that the absolute number of positive cells per million correlates very well with the occurrence of symptomatic CMV disease.

Tài liệu tham khảo

Cheeseman SH, Rubin RH, Stewart JA, Tolkoff-Rubin NE, Cosimi AB, Cantell K, Gilbert J, Winkle S, Herrin JT, Black PH, Russell PS, Hirsch MS (1979) Controlled clinical trial of prophylactic human leukocyte interferon in renal transplantation: effect on cytomegalovirus and herpes simplex virus infections. N Engl J Med 300: 1345–1349

Dussaix E, Wood C (1989) Cytomegalovirus infection in pediatric liver recipients. Transplantation 48: 272–274

Fryd DS, Peterson PK, Fergusson RM, Simmons RL, Balfour HH (1980) Cytomegalovirus as a risk factor in renal transplantation. Transplantation 30: 436–439

Griffiths PD, Panjwani DD, Stirk PR, Ball MG, Ganczakowski M, Blacklock HA, Prentice HG (1984) Rapid diagnosis of cytomegalovirus infection in immunocompromised patients by detection of early antigen fluorescent foci. Lancet II: 1242–1245

Johnson PC, Lewis RM, Golden DL, Oefinger PE, Buren CT van, Kerman RH, Kahan BD (1988) The impact of cytomegalovirus infection on seronegative recipients of seropositive donor kidneys versus seropositive recipients treated with cyclosporine-prednisone immunosuppression. Transplantation 45: 116–121

Marker SC, Howard RJ, Simmons RL, Kalìs JM, Connelly DP, Najarian JS, Balfour HH Jr (1981) Cytomegalovirus infection: a quantative prospective study of three hundred twenty consecutive renal transplants. Surgery 89: 660–671

Peterson PK, Balfour HH, Marker SC (1980) Cytomegalovirus in renal allograft recipients: a prospective study of the clinical features, risk factors and impact on renal transplantation. Medicine 59: 283–300

Ploem JS, Driel-Kulker AMJ van, Goyarts-Veldstra L, Ploem-Zaayer JJ, Verwoerd NP, Zwan M van der (1986) Image analysis combined with quantitative cytochemistry. Results and instrumental developments for cancer diagnosis. Histochemistry 84: 549–555

Rubin RH, Tolkoff-Rubin NE, Oliver D, Hamilton J, Betts RF, Pass RF, Hillis W, Szmuness J, Farrell ML, Hirsch MS (1985) Multicenter sero epidemiologic study of the impact of cytomegalovirus infection on renal transplants. Transplantation 40: 243–249

Simmons RL, Weil R, Tallent MB, Kjellstrand CM, Najarian JS (1970) Do mild infections trigger the rejection of renal allografts. Transplant Proc 2: 419

Smiley ML, Woldaver CG, Grossman RA, Barker CF, Perloff LJ, Tustin NB, Starr SE, Plothin SA, Friedman HM (1985) The role of pretransplant immunity in protection from cytomegalovirus disease following renal transplantation. Transplantation 40- 157–161

Whelchel JD, Pass RF, Diethelm AG, Whitley RJ, Alford CA (1979) Effect of primary and recurrent cytomegalovirus infections upon graft and patient survival after renal transplantation. Transplantation 28: 443–446