Survey on the distribution of the gene 4 alleles of human rotaviruses by polymerase chain reaction

Epidemiology and Infection - Tập 112 Số 3 - Trang 615-622 - 1994
Haotian Wu1, K. Taniguchi2, F. Wakasugi2, Susumu Ukae3, S. Chiba3, Mitsuaki OHSETO4, A. Hasegawa5, Tomoko Urasawa2, Shozo Urasawa2
1Department of Hygiene, School of Medicine, Sapporo Medical University, Japan
2Department of Hygiene, School of Medicine, Sapporo Medical University, Sapporo 060, Japan
3Department of Pediatrics, School of Medicine, Sapporo Medical University. Sapporo 060, Japan
4Ehime Institute of Public Health, Matsuyama, Japan
5Department of Epidemiology, National Institute of Health, Tokyo, Japan

Tóm tắt

SUMMARYThe presence of six gene 4 alleles (or VP4 genotypes) in human rotaviruses has been recognized. Using 16 representative cultivable human rotavirus strains, we confirmed the specificity of VP4 genotyping by polymerase chain reaction (PCR) using the nested oligonucleotides specific to each of the four representative gene 4 alleles. Using the PCR. we surveyed the gene 4 alleles of 199 human rotaviruses in stools collected in Japan and Thailand. Strains with the gene 4 allele, corresponding to P1A serotype. were shown to be the most prevalent, but two strains with P2 gene 4 allele and one strain with P3 gene 4 allele were detected in Thailand and in Japan, respectively.

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Tài liệu tham khảo

Sereno, The outer capsid protein VP4 of murine rotavirus Eb represents a tentative new P type, Virology.

Taniguchi, Species specificity and interspecies relatedness in VP4 genotypes demonstrated by VP4 sequence analysis of equine, feline and canine rotavirus strains, Virology.

10.1006/viro.1993.1280

10.1099/0022-1317-74-6-1215

Taniguchi, 1988, Identification of cross-reactive and serotype-2 specific neutralization epitopes on VP3 of human rotavirus, J Virol, 62, 2421, 10.1128/JVI.62.7.2421-2426.1988

Taniguchi, 1992, Identification of human and bovine rotavirus serotypes by polymerase chain reaction, Epidemiol Infect, 28, 276

Taniguchi, 1990, Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup I specificity and long RNA pattern: implication for new G serotype specificity, J Virol, 64, 5640, 10.1128/JVI.64.11.5640-5644.1990

Li, 1993, Human rotavirus K8 strain represents a new VP4 serotype, J Virol, 67, 617, 10.1128/JVI.67.1.617-620.1993

10.1073/pnas.87.18.7155

10.1093/infdis/155.6.1159

10.1099/0022-1317-73-8-1939

Gorziglia, 1988, Sequence of the fourth gene of human rotaviruses recovered from asymptomatic or symptomatic infections, J Virol, 62, 2978, 10.1128/JVI.62.8.2978-2984.1988

Gouvea, 1990, Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens, J Clin Microbiol, 28, 276, 10.1128/JCM.28.2.276-282.1990

10.1006/viro.1993.1271

Woods, 1992, Distribution of serotypes of human rotavirus in different populations, J Clin Microbiol, 30, 781, 10.1128/JCM.30.4.781-785.1992

10.1073/pnas.82.24.8701

Steele, 1993, Distribution of VP4 gene alleles in human rotaviruses by using probes to the hyperdivergent region of the VP4 gene, J Clin Microbiol, 31, 1735, 10.1128/JCM.31.7.1735-1740.1993

Matsui, 1989, Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to the heterotypic neutralization domain of VP7 and VP8 fragment of VP4, J Clin Microbiol, 27, 780, 10.1128/JCM.27.4.780-782.1989

10.1016/0042-6822(91)90691-4

Larralde, 1991, Serotype-specific epitope(s) present on the VP8 subunit of rotavirus VP4 protein, J Virol, 65, 3213, 10.1128/JVI.65.6.3213-3218.1991

Taniguchi, 1989, Complete nucleotide sequence of the gene encoding VP4 of a human rotavirus (strain K8) which has unique VP4 neutralization epitopes, J Virol, 63, 4101, 10.1128/JVI.63.9.4101-4106.1989

Urasawa, 1990, Antigenic and genetic analyses of human rotavirus with dual subgroup specificity, J Clin Microbiol, 28, 2837, 10.1128/JCM.28.12.2837-2841.1990

10.1006/viro.1993.1540

10.1093/infdis/166.2.227

10.1007/BF01316680

Estes, 1989, Rotavirus gene structure and function, Microbiol Rev, 53, 410, 10.1128/MMBR.53.4.410-449.1989

Gentsch, 1992, Identification of group A rotavirus gene 4 types by polymerase chain reaction, J Clin Microbiol, 30, 1365, 10.1128/JCM.30.6.1365-1373.1992

10.1073/pnas.85.3.645

10.1093/infdis/160.1.44

10.1093/infdis/153.5.815

Kapikian, 1990, Virology, 1353

Beards, 1992, A serotype 10 human rotavirus, J Clin Microbiol, 30, 1432, 10.1128/JCM.30.6.1432-1435.1992

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Epidemiology and Infection

Tập 112 Số 3

615-622

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