Sulphate assimilation under Cd<sup>2+</sup> stress in <i>Physcomitrella patens </i>– combined transcript, enzyme and metabolite profiling

Plant, Cell and Environment - Tập 29 Số 9 - Trang 1801-1811 - 2006
Michael Rother1, Gerhard Krauss, Gregor Grass, Dirk Wesenberg
1Martin Luther University Halle-Wittenberg, Departments of Biochemistry/Biotechnology, Halle, Germany.

Tóm tắt

ABSTRACTCd2+ causes disturbance of metabolic pathways through severe damage on several levels. Here we present a comprehensive study of Cd2+‐mediated effects on transcript, enzyme and metabolite levels in a plant without phytochelatin (PC). The moss Physcomitrella patens (Hedw.) B.S.G. was stressed with up to 10 µm Cd2+ to investigate the regulation of gene transcription and activities of enzymes involved in the assimilatory sulphate reduction pathway and in glutathione biosynthesis. Real‐time PCR, specific enzyme assays as well as thiol peptide profiling techniques were applied. Upon supplementation of 10 µm Cd2+, the moss showed a more than fourfold increase in expression of genes encoding ATP sulphurylase (ATPS), adenosylphosphosulphate reductase, phosphoradenosylphosphorsulphate reductase, sulphite reductase (SiR) and γ‐glutamyl cysteine synthetase (γ‐ECS). Likewise, elevated enzyme activities of γ‐ECS and glutathione synthetase were observed. Contrarily, activity of O‐acetylserine (thiol) lyase (OAS‐TL), responsible for biosynthesis of cysteine, was diminished. At the metabolite level, nearly doubling of intracellular cysteine and glutathione content was noted, while the moss did not produce any detectable amounts of PCs. These results suggest a Cd2+‐induced activation of the assimilatory sulphate reduction pathway as well as of glutathione biosynthesis on different levels of regulation.

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