Successful Application of Alpha Lipoic Acid Niosomal Formulation in Cerebral Ischemic Reperfusion Injury in Rat Model

Advanced Pharmaceutical Bulletin - Tập 12 Số 3 - Trang 541-549 - 2022
Mohammad Amin Raeisi Estabragh1,2, Abbas Pardakhty1, Saeid Ahmadzadeh3, Shahriar Dabiri4, Hamid Reza Mollaei4, Mohammad Mehdi Oloumi5
1Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
2Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran
3Pharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical Sciences, Kerman, Iran
4Pathology and Stem Cell Research Center, Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
5Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

Tóm tắt

Purpose: Free radicals such as hydroxyl and peroxide are contributing factors to neuronaldestruction in cerebral ischemia. Alpha-lipoic acid (ALA) is one of the potent knownantioxidants. Preparation of ALA niosomes allows IV injection and can increase bioavailabilityand penetration into the central nervous system (CNS).Methods: Film hydration method was used to prepare different niosomes composed of Span®,Tween®, and cholesterol at different molar ratio. ALA and niosome-forming compounds weredissolved in chloroform, before removing the organic solvent by rotary evaporator. Animalswere randomly divided into four groups: Sham, control group, intravenous (IV) injection ofempty niosomes plus intraperitoneal (IP) injection of ALA solution, and finally, IV injectionof ALA niosomes. Rats were subjected to deep anesthesia before inducing cerebral ischemia,then, their internal common carotid arteries were clamped for 15 min and reperfusion wasdone for 30 min. Niosomal ALA was injected intravenously just before declamping.Results: Mean volume diameter of the prepared niosomes was between 4.36 ± 0.82 and 19.95± 1.21 μm in different formulations. Encapsulation efficiency percent (EE%) of ALA in theselected formulation, Span60/Tween60/cholesterol (35:35:30 molar ratio), was 94.5 ± 0.2, and59.27 ± 5.61% of ALA was released after 4h. In the niosomal group, the rate of reduction incomplications of cerebral ischemia such as histopathologic changes and acute damage (fromscore 3 to 1) in CNS was higher than other groups.Conclusion: The obtained results show that niosomes can be used as effective drug deliverysystems for ALA in cerebral ischemia.

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Advanced Pharmaceutical Bulletin

Tập 12 Số 3

541-549

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