Phân tích đặc hiệu cơ chất của phức hợp kinase protein Dbf2-Mob1 qua lựa chọn thư viện peptide và sàng lọc mảng proteome

BMC Biochemistry - Tập 6 Số 1 - 2005
Angie S. Mah1, Andrew E. H. Elia2, Geeta Devgan3, Jason Ptacek4, Mike Schutkowski5, M Snyder3, Michael B. Yaffe6, Raymond J. Deshaies7
1Department of Biology, California Institute of Technology, Pasadena, CA, 91125, USA
2Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139 USA
3Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
4Department of Molecular Biophysics & Biochemistry, Yale University, New Haven, CT, 06520, USA
5JPT Peptide Technologies GmbH, Invalidenstrasse 130, 10115, Berlin, Germany, USA
6Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139 USA
7Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA

Tóm tắt

Tóm tắt Đặt bối cảnh

Mạng lưới thoát mitotic (MEN) là một nhóm protein tạo thành một chuỗi tín hiệu thiết yếu để các tế bào thoát khỏi quá trình phân bào trong Saccharomyces cerevisiae. MEN cũng đã được liên quan đến vai trò trong phân chia tế bào. Hai thành phần của con đường tín hiệu này là kinase protein Dbf2 và đối tác liên kết của nó cần thiết cho hoạt động kinase, Mob1. Các thành phần của MEN hoạt động phía trên Dbf2-Mob1 đã được xác định, nhưng các cơ chất sinh lý cho Dbf2-Mob1 vẫn cần được xác định.

Từ khóa

#Dbf2-Mob1 #kinase protein #phân chia tế bào #tín hiệu sinh học #cơ chất phosphoryl hóa

Tài liệu tham khảo

D'Amours D, Amon A: At the interface between signaling and executing anaphase – Cdc14 and the FEAR network. Genes Dev. 2004, 18 (21): 2581-2595. 10.1101/gad.1247304.

Schwab M, Lutum AS, Seufert W: Yeast Hct1 is a regulator of Clb2 cyclin proteolysis. Cell. 1997, 90 (4): 683-693. 10.1016/S0092-8674(00)80529-2.

Visintin R, Craig K, Hwang ES, Prinz S, Tyers M, Amon A: The phosphatase Cdc14 triggers mitotic exit by reversal of Cdk-dependent phosphorylation. Mol Cell. 1998, 2 (6): 709-718. 10.1016/S1097-2765(00)80286-5.

Zachariae W, Schwab M, Nasmyth K, Seufert W: Control of cyclin ubiquitination by CDK-regulated binding of Hct1 to the anaphase promoting complex. Science. 1998, 282 (5394): 1721-1724. 10.1126/science.282.5394.1721.

Jaspersen SL, Charles JF, Morgan DO: Inhibitory phosphorylation of the APC regulator Hct1 is controlled by the kinase Cdc28 and the phosphatase Cdc14. Curr Biol. 1999, 9 (5): 227-236. 10.1016/S0960-9822(99)80111-0.

Moll T, Tebb G, Surana U, Robitsch H, Nasmyth K: The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5. Cell. 1991, 66 (4): 743-758. 10.1016/0092-8674(91)90118-I.

Shou W, Seol JH, Shevchenko A, Baskerville C, Moazed D, Chen ZW, Jang J, Charbonneau H, Deshaies RJ: Exit from mitosis is triggered by Tem1- dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Cell. 1999, 97 (2): 233-244. 10.1016/S0092-8674(00)80733-3.

Visintin R, Hwang ES, Amon A: Cfi1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus. Nature. 1999, 398 (6730): 818-823. 10.1038/19775.

Stegmeier F, Amon A: Closing Mitosis: The Functions of the Cdc14 Phosphatase and Its Regulation. Annu Rev Genet. 2004, 38: 203-232. 10.1146/annurev.genet.38.072902.093051.

Azzam R, Chen SL, Shou W, Mah AS, Alexandru G, Nasmyth K, Annan RS, Carr SA, Deshaies RJ: Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus. Science. 2004, 305 (5683): 516-519. 10.1126/science.1099402.

Stegmeier F, Visintin R, Amon A: Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase. Cell. 2002, 108 (2): 207-220. 10.1016/S0092-8674(02)00618-9.

Sullivan M, Uhlmann F: non-proteolytic function of separase links the onset of anaphase to mitotic exit. Nat Cell Biol. 2003, 5 (3): 249-254. 10.1038/ncb940.

Stegmeier F, Huang J, Rahal R, Zmolik J, Moazed D, Amon A: The replication fork block protein Fob1 functions as a negative regulator of the FEAR network. Curr Biol. 2004, 14 (6): 467-480. 10.1016/j.cub.2004.03.009.

Bardin AJ, Amon A: Men and sin: what's the difference?. Nat Rev Mol Cell Biol. 2001, 2 (11): 815-826. 10.1038/35099020.

Pereira G, Hofken T, Grindlay J, Manson C, Schiebel E: The Bub2p spindle checkpoint links nuclear migration with mitotic exit. Mol Cell. 2000, 6 (1): 1-10. 10.1016/S1097-2765(00)00002-2.

Bardin AJ, Visintin R, Amon A: A mechanism for coupling exit from mitosis to partitioning of the nucleus. Cell. 2000, 102 (1): 21-31. 10.1016/S0092-8674(00)00007-6.

Mah AS, Jang J, Deshaies RJ: Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex. Proc Natl Acad Sci USA. 2001, 98 (13): 7325-7330. 10.1073/pnas.141098998.

Xu S, Huang HK, Kaiser P, Latterich M, Hunter T: Phosphorylation and spindle pole body localization of the Cdc15p mitotic regulatory protein kinase in budding yeast. Curr Biol. 2000, 10 (6): 329-332. 10.1016/S0960-9822(00)00382-1.

Frenz LM, Lee SE, Fesquet D, Johnston LH: The budding yeast Dbf2 protein kinase localises to the centrosome and moves to the bud neck in late mitosis. J Cell Sci. 2000, 113 (Pt 19): 3399-3408.

Visintin R, Amon A: Regulation of the mitotic exit protein kinases Cdc15 and Dbf2. Mol Biol Cell. 2001, 12 (10): 2961-2974.

Hwa Lim H, Yeong FM, Surana U: Inactivation of mitotic kinase triggers translocation of MEN components to mother-daughter neck in yeast. Mol Biol Cell. 2003, 14 (11): 4734-4743. 10.1091/mbc.E03-04-0238.

Yoshida S, Toh-e A: Regulation of the localization of Dbf2 and Mob1 during cell division of Saccharomyces cerevisiae. Genes Genet Syst. 2001, 76 (2): 141-147. 10.1266/ggs.76.141.

Luca FC, Mody M, Kurischko C, Roof DM, Giddings TH, Winey M: Saccharomyces cerevisiae Mob1p is required for cytokinesis and mitotic exit. Mol Cell Biol. 2001, 21 (20): 6972-6983. 10.1128/MCB.21.20.6972-6983.2001.

Lippincott J, Shannon KB, Shou W, Deshaies RJ, Li R: The Tem1 small GTPase controls actomyosin and septin dynamics during cytokinesis. J Cell Sci. 2001, 114 (Pt 7): 1379-1386.

Frenz LM, Lee SE, Fesquet D, Johnston LH: The budding yeast Dbf2 protein kinase localises to the centrosome and moves to the bud neck in late mitosis. J Cell Sci. 2000, 113 (Pt 19): 3399-3408.

Obata T, Yaffe MB, Leparc GG, Piro ET, Maegawa H, Kashiwagi A, Kikkawa R, Cantley LC: Peptide and protein library screening defines optimal substrate motifs for AKT/PKB. J Biol Chem. 2000, 275 (46): 36108-36115. 10.1074/jbc.M005497200.

Songyang Z, Blechner S, Hoagland N, Hoekstra MF, Piwnica-Worms H, Cantley LC: Use of an oriented peptide library to determine the optimal substrates of protein kinases. Curr Biol. 1994, 4 (11): 973-982. 10.1016/S0960-9822(00)00221-9.

Hunter T, Plowman GD: The protein kinases of budding yeast: six score and more. Trends Biochem Sci. 1997, 22 (1): 18-22. 10.1016/S0968-0004(96)10068-2.

Schutkowski M, Reimer U, Panse S, Dong L, Lizcano JM, Alessi DR, Schneider-Mergener J: High-Content Peptide Microarrays for Deciphering Kinase Specificity and Biology. Angew Chem. 2004, 116: 2725-2728. 10.1002/ange.200453900.

Millward TA, Heizmann CW, Schafer BW, Hemmings BA: Calcium regulation of Ndr protein kinase mediated by S100 calcium-binding proteins. EMBO J. 1998, 17 (20): 5913-5922. 10.1093/emboj/17.20.5913.

Songyang Z, Cantley LC: The use of peptide library for the determination of kinase peptide substrates. Methods Mol Biol. 1998, 87: 87-98.

Yaffe MB, Schutkowski M, Shen M, Zhou XZ, Stukenberg PT, Rahfeld JU, Xu J, Kuang J, Kirschner MW, Fischer G: Sequence-specific and phosphorylation-dependent proline isomerization: a potential mitotic regulatory mechanism. Science. 1997, 278 (5345): 1957-1960. 10.1126/science.278.5345.1957.

Panse S, Dong L, Carus R, Schutkowski M, Reimer U, Schneider-Mergener J: Profiling of generic and anti-phosphopeptide antibodies and kinases with peptide microarrays using radioactive and fluorescence-based assays. Molecular Diversity. 2004, 8: 291-299. 10.1023/B:MODI.0000036240.39384.eb.

Rychlewski L, Kschischo M, Dong L, Schutkowski M, Reimer U: Target specificity analysis of the Abl kinase using peptide microarray data. J Mol Biol. 2004, 336: 307-311. 10.1016/j.jmb.2003.12.052.

Zhu H, Snyder M: Protein arrays and microarrays. Curr Opin Chem Biol. 2001, 5 (1): 40-45. 10.1016/S1367-5931(00)00170-8.