Strategies for microsatellite isolation: a review

Molecular Ecology - Tập 11 Số 1 - Trang 1-16 - 2002
Lorenzo Zane1, Luca Bargelloni1, Tomaso Patarnello2,1
1Dipartimento di Biologia, Università di Padova, Via Ugo Bassi 58/B, 35121 Padova, Italy
2Agripolis/Facoltà di Veterinaria, Università di Padova 35020, Legnaro (Pd), Italy

Tóm tắt

AbstractIn the last few years microsatellites have become one of the most popular molecular markers used with applications in many different fields. High polymorphism and the relative ease of scoring represent the two major features that make microsatellites of large interest for many genetic studies. The major drawback of microsatellites is that they need to be isolated de novo from species that are being examined for the first time. The aim of the present paper is to review the various methods of microsatellite isolation described in the literature with the purpose of providing useful guidelines in making appropriate choices among the large number of currently available options. In addition, we propose a fast and easy protocol which is a combination of different published methods.

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Molecular Ecology

Tập 11 Số 1

1-16

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