Stem cell patterns in cell lines derived from head and neck squamous cell carcinoma

Journal of Oral Pathology and Medicine - Tập 36 Số 10 - Trang 594-603 - 2007
Lisa J. Harper1, Kim Piper, John Common, Farida Fortune, Ian C. Mackenzie
1Institute of Cell and Molecular Science, Queen Mary's School of Medicine and Dentistry, Whitechapel, London, UK.

Tóm tắt

The initiation, growth, recurrence and metastasis of solid tumours, including squamous cell carcinoma of the head and neck region, have been related to the behaviour of a small subpopulation of ‘tumour‐initiating’ cells. Cells with stem cell characteristics have also been identified in cell lines derived from cancers and the aim of the present work was to extend examination of such cells. Established cell lines were examined for their patterns of colony morphologies and staining, the presence of a Hoechst dye‐excluding ‘side population’, expression of the putative stem cell markers CD44, CD133 and CD29, and their ability to grow as ‘cancer spheroids’. Two cell lines, CaLH2 and CaLH3, recently generated from HNSCC tumour biopsies, were similarly examined. All cell lines showed a holoclone/meroclone/paraclone series of colony morphologies and cell sorting indicated that CD44 marker expression was related to clonogenicity. FACS analysis after exposure to Hoechst dye indicated that the CA1, H357 and UK1 cell lines contain a dye‐excluding ‘side population’, a property associated with stem‐like subpopulations. When held in suspension, all cell lines formed spheroids that could be re‐passaged. These observations indicate that cell lines derived from HNSCC contain cells with stem cell properties and that such cell lines may provide experimental systems relevant to the behaviour of stem cells present in the tumours of origin and to their responses to therapy.

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Thông tin xuất bản

Journal of Oral Pathology and Medicine

Tập 36 Số 10

594-603

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