Starch content differences between two sweet potato accessions are associated with specific changes in gene expression

Springer Science and Business Media LLC - Tập 18 - Trang 613-625 - 2018
Songtao Yang1, Xiaojing Liu2, Shuai Qiao1, Wenfang Tan1, Ming Li3, Junyan Feng3, Cong Zhang3, Xiang Kang2, Tianbao Huang4, Youlin Zhu2, Lan Yang2, Dong Wang2
1Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China
2Key Laboratory of Molecular Biology and Gene Engineering in Jiangxi Province, College of Life Science, Nanchang University, Nanchang, China
3Institute of Biotechnology and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu, China
4Jiangxi Institute of Red Soil, Nanchang, China

Tóm tắt

Sweet potato (Ipomoea batatas (L.) Lam.) is one of the most important root crops in the world. Initial formation and development of storage roots (SRs) are key factors affecting its yields. In order to study the molecular mechanism and regulatory networks of the SRs development process, we have analyzed root transcriptomes between the high and low starch content sweet potato accessions at three different developmental stages. In this study, we assembled 46,840 unigenes using Illumina paired-end sequencing reads and identified differentially expressed genes (DEGs) between two accessions. The numbers of DEGs were increased with the development of SRs, indicating that the difference between two accessions is enlarging with the maturation. DEGs were mainly enriched in starch biosynthesis, plant hormones regulatory, and genetic information processing pathways. Then, expression patterns of DEGs that are most significant and starch biosynthesis related were validated using qRT-PCR. Our results provide valuable resources to future study on molecular mechanisms of SRs development and candidate genes for starch content improvement in sweet potato.

Tài liệu tham khảo

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