Staphylococcal enterotoxin B influences the DNA methylation pattern in nasal polyp tissue: a preliminary study

Allergy, Asthma & Clinical Immunology - Tập 9 - Trang 1-5 - 2013
Claudina A Pérez-Novo1, Yuan Zhang2,3, Simon Denil4, Geert Trooskens4, Tim De Meyer4, Wim Van Criekinge4, Paul Van Cauwenberge1, Luo Zhang2, Claus Bachert1,5
1Upper Airways Research Laboratory, Department of Otorhinolaryngology, Ghent University Hospital, Ghent, Belgium
2Department of Otolaryngology–Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, PR China
3Key Laboratory of Otolaryngology, Head and Neck Surgery (Ministry of Education of China), Beijing Institute of Otorhinolaryngology, Beijing, PR China
4Department of Mathematical Modelling, Statistics and Bioinformatics, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
5Karolinska Institutet, Division of ENT Diseases, CLINTEC, Stockholm, Sweden

Tóm tắt

Staphylococcal enterotoxins may influence the pro-inflammatory pattern of chronic sinus diseases via epigenetic events. This work intended to investigate the potential of staphylococcal enterotoxin B (SEB) to induce changes in the DNA methylation pattern. Nasal polyp tissue explants were cultured in the presence and absence of SEB; genomic DNA was then isolated and used for whole genome methylation analysis. Results showed that SEB stimulation altered the methylation pattern of gene regions when compared with non stimulated tissue. Data enrichment analysis highlighted two genes: the IKBKB and STAT-5B, both playing a crucial role in T- cell maturation/activation and immune response.

Tài liệu tham khảo

Bachert C, Van SK, Zhang N, Holtappels G, Cattaert T, Maus B, Buhl R, Taube C, Korn S, Kowalski M: Specific IgE against Staphylococcus aureus enterotoxins: an independent risk factor for asthma. J Allergy Clin Immunol. 2012, 130: 376-381. 10.1016/j.jaci.2012.05.012. Fokkens WJ, Lund VJ, Mullol J, Bachert C, Alobid I, Baroody F, Cohen N, Cervin A, Douglas R, Gevaert P: European position paper on rhinosinusitis and nasal polyps. Rhinol Suppl. 2012, 2012: 3-298. Bierne H, Hamon M, Cossart P: Epigenetics and bacterial infections. Cold Spring Harb Perspect Med. 2012, 2: a010272- Patou J, Gevaert P, Van ZT, Holtappels G, Van CP, Bachert C: Staphylococcus aureus enterotoxin B, protein A, and lipoteichoic acid stimulations in nasal polyps. J Allergy Clin Immunol. 2008, 121: 110-115. 10.1016/j.jaci.2007.08.059. Hardcastle TJ, Kelly KA: baySeq: empirical Bayesian methods for identifying differential expression in sequence count data. BMC Bioinforma. 2010, 11: 422-10.1186/1471-2105-11-422. Langmead B, Trapnell C, Pop M, Salzberg SL: Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009, 10: R25-10.1186/gb-2009-10-3-r25. D'Eustachio P: Reactome knowledgebase of human biological pathways and processes. Methods Mol Biol. 2011, 694: 49-61. 10.1007/978-1-60761-977-2_4. Shiomori T, Yoshida S, Miyamoto H, Makishima K: Relationship of nasal carriage of Staphylococcus aureus to pathogenesis of perennial allergic rhinitis. J Allergy Clin Immunol. 2000, 105: 449-454. 10.1067/mai.2000.104256.
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Allergy, Asthma & Clinical Immunology

Tập 9

1-5

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