H.F. Kauffman1, S. van der Heide1, Siem van der Laan1, H. Hovenga1, F Beaumont2, K. de Vries1
1Allergology and
2Pulmonology, Clinic for Internal Medicine, State University Hospital, Groningen, The Netherlands
Tóm tắt
During cultivation of <i>Aspergillus fumigatus </i>a rapid liberation of IgE-binding components was found reaching maximum values during the logarithmic phase of growth (phase I). After a fall in IgE-binding titers during phase II, appearance of additional IgE-binding components was noted during the period of lysis of the microorganism (phase III). These latter allergenic components are different from the phase I IgE-binding components, as was shown by crossed-inhibition studies. The number of precipitating antigenic components was not related with the corresponding IgE-binding titers and showed an increase during all phases of growth. The rapid changes in both IgE- and IgG-binding properties and the discrepancies between precipitating properties and IgE binding are discussed in relation to standardization and quality control of aspergillus extracts.
