Standardisierte mikroRNA-Analytik — die große Chance für Biomarkerstudien?

BIOspektrum - 2020
Maria Fauth1, Meike J. Saul1
1Fachbereich Biologie, Technische Universität Darmstadt, Darmstadt, Deutschland

Tóm tắt

AbstractCirculating microRNAs (miRs) represent promising diagnostic and prognostic biomarkers for various diseases. Despite the high number of biomarker studies, the results of these studies are hardly reproducible. This makes it difficult to transfer the results into clinical application. In this context, acceptance criteria for the quantification of miRs by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) may help to standardize miR analysis and improve reproducibility.

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Tài liệu tham khảo

Pardini B, Sabo AA, Birolo G et al. (2019) Noncoding RNAs in extracellular fluids as cancer biomarkers: The New Frontier of Liquid Biopsies. Cancers 11:1170

Saul MJ, Emmerich AC, Steinhilber D et al. (2019) Regulation of eicosanoid pathways by microRNAs. Front Pharmacol 10:824

Mitchell PS, Parkin RK, Kroh EM et al. (2008) Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci USA 105:10513–10518

Witwer KW (2015) Circulating microRNA biomarker studies: pitfalls and potential solutions. Clin Chem 61:56–63

Fauth M, Hegewald AB, Schmitz L et al. (2019) Validation of extracellular miRNA quantification in blood samples using RT-qPCR. FASEB Bioadv 1:481–492

Wang K, Yuan Y, Cho JH et al. (2012) Comparing the microRNA spectrum between serum and plasma. PLoS One 7:e41561

Makhro A, Huisjes R, Verhagen LP et al. (2016) Red cell properties after different modes of blood transportation. Front Physiol 7:288

McDonald JS, Milosevic D, Reddi HV et al. (2011) Analysis of circulating microRNA: preanalytical and analytical challenges. Clin Chem 57:833–840

Kim YK, Yeo J, Kim B et al. (2012) Short structured RNAs with low GC content are selectively lost during extraction from a small number of cells. Mol Cell 46:893–895

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