Stable transformation of Sorghum bicolor protoplasts with chimeric neomycin phosphotransferase II and β-glucuronidase genes
Tóm tắt
Từ khóa
Tài liệu tham khảo
Battraw MJ, Hall TC (1990) Histochemical analysis of CaMV 35S promoter-β-glucuronidase gene expression in transgenic rice plants. Plant Mol Biol 15:527–538
Callis J, Fromm M, Walbot V (1987) Introns increase gene expression in cultured maize cells. Gene Dev 1:1183–1200
Chourey PS, Sharpe DZ (1985) Callus formation from protoplasts of Sorghum cell suspension cultures. Plant Sci 39:171–175
Chourey PS, Zurawski DB (1981) Callus formation from protoplasts of a maize cell culture. Theor Appl Genet 59:341–344
Fromm M, Taylor LP, Walbot V (1985) Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc Natl Acad Sci USA 82:5824–5828
Fromm ME, Taylor LP, Walbot V (1986) Stable transformation of maize after gene transfer by electroporation. Nature 319:791–793
Gordon-Kamm WJ, Spencer TM, Mangano ML, Adams TR, Daines RJ, Start WG, O'Brien JV, Chambers SA, Adams WR, Willetts NG, Rice TB, Mackey CJ, Krueger RW, Kausch AP, Lemaux PG (1990) Transformation of maize cells and regeneration of fertile transgenic plants. Plant cell 2:603–618
Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387–405
Jefferson RA, Kavanagh TA, Beven MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907
Krens FA, Molendijk L, Wullems GJ, Schilperoort RA (1982) In vitro transformation of plant protoplasts with Ti-plasmid DNA. Nature 296:72–74
Labarca C, Paigen K (1980) A simple, rapid, and sensitive DNA assay procedure. Anal Biochem 102:344–352
Laurie DA, Bennett MD (1985) Nuclear DNA content in the genera Zea and Sorghum. Intergeneric, interspecific, and intraspecific variation. Heredity 55:307–313
Lyznik LA, Ryan RD, Ritchie SW, Hodges TK (1989) Stable cotransformation of maize protoplasts with gusA and neo genes. Plant Mol Biol 13:151–161
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY
Paszkowski J, Shillito RD, Saul M, Mandak V, Hohn T, Hohn B, Potrykus I (1984) Direct gene transfer to plants. EMBO J 3:2717–2722
Potrykus I (1990) Gene transfer to cereals: an assessment. Bio/Technol 8:535–542
Potrykus I, Saul MW, Petruska J, Paszkowski J, Shillito RD (1985) Direct gene transfer to cells of a graminaceous monocot. Mol Gen Genet 199:183–188
Potter H, Weir L, Leder P (1984) Enhancer-dependent expression of human χ immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation. Proc Natl Acad Sci USA 81:7161–7165
Reed KC (1988) Evaluation of alkaline Southern transfers. Molecular Biology Rep 4:3–4
Reed KC, Mann DA (1985) Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res 13:7207–7221
Reiss B, Sprengel R, Will H, Schaller H (1984) A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extracts. Gene 30:211–218
Rhodes CA, Pierce DA, Mettler IJ, Mascarenhas D, Detmer JJ (1988) Genetically transformed maize plants from protoplasts. Science 240:204–207
Schocher RJ, Shillito RD, Saul MW, Paszkowski J, Potrykus I (1986) Cotransformation of unlinked foreign genes into plants by direct gene transfer. Bio/Technol 4:1093–1096
Schreier PH, Seftor EA, Schell J, Bohnert HJ (1985) The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts. EMBO J 4:25–32
Shillito RD, Saul MW, Paszkowski J, Müller M, Potrykus I (1985) High efficiency direct gene transfer to plants. Bio/Technol 3:1099–1103
Shimamoto K, Terada RT, Izawa T, Fujimoto H (1989) Fertile transgenic rice plants regenerated from transformed protoplasts. Nature 338:274–276
Taylor B, Powell A (1982) Isolation of plant DNA and RNA. Focus 4:4–6
Uchimiya H, Fushimi T, Hashimoto H, Harada H, Syono K, Sugawara Y (1986 a) Expression of a foreign gene in callus derived from DNA-treated protoplasts of rice (Oryza sativa L.). Mol Gen Genet 204:204–207
