Stability issues of RT‐PCR testing of SARS‐CoV‐2 for hospitalized patients clinically diagnosed with COVID‐19

Journal of Medical Virology - Tập 92 Số 7 - Trang 903-908 - 2020
Yafang Li1, Lin Yao2, Jiawei Li3, Lei Chen1, Yiyan Song4, Zhifang Cai5, Chunhua Yang1
1Department of Intensive Care Unit, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China
2Department of Operation and Management, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
3Natural Language Processing Group, iFLYTEK Research South China, Guangzhou, China
4Department of Clinical Medicine, Zhongshan School of Medicine, Guangzhou, China
5Department of Respiratory, Hankou Hospital of Wuhan, Wuhan, China

Tóm tắt

AbstractIn this study, we collected a total of 610 hospitalized patients from Wuhan between February 2, 2020, and February 17, 2020. We reported a potentially high false negative rate of real‐time reverse‐transcriptase polymerase chain reaction (RT‐PCR) testing for SARS‐CoV‐2 in the 610 hospitalized patients clinically diagnosed with COVID‐19 during the 2019 outbreak. We also found that the RT‐PCR results from several tests at different points were variable from the same patients during the course of diagnosis and treatment of these patients. Our results indicate that in addition to the emphasis on RT‐PCR testing, clinical indicators such as computed tomography images should also be used not only for diagnosis and treatment but also for isolation, recovery/discharge, and transferring for hospitalized patients clinically diagnosed with COVID‐19 during the current epidemic. These results suggested the urgent needs for the standard of procedures of sampling from different anatomic sites, sample transportation, optimization of RT‐PCR, serology diagnosis/screening for SARS‐CoV‐2 infection, and distinct diagnosis from other respiratory diseases such as fluenza infections as well.

Từ khóa


Tài liệu tham khảo

China CDC.Distribution of Chinese and global COVID‐19 outbreak daily updated by China CDC;2020.http://2019ncov.chinacdc.cn/2019‐nCoV/index.html

10.2807/1560-7917.ES.2020.25.3.2000045

China National Health Commission, 2020, Diagnosis and Treatment of Pneumonitis Caused by New Coronavirus (Trial Version 5)

10.1148/radiol.2020200343

Huang P, 2020, Use of chest CT in combination with negative RT‐PCR assay for the 2019 novel coronavirus but high clinical suspicion, Radiology, 200330

Qian M, 2020, Understanding the influencing factors of nucleic acid detection of 2019 novel coronavirus, Chin J Lab Med

Thông tin
Thông tin xuất bản

Journal of Medical Virology

Tập 92 Số 7

903-908

Thông tin tác giả