Sphingosine 1‐phosphate released from platelets during clotting accounts for the potent endothelial cell chemotactic activity of blood serum and provides a novel link between hemostasis and angiogenesis

Wiley - Tập 14 Số 14 - Trang 2255-2265 - 2000
Denis English1,2, Zachary Welch3, A. Thomas Kovala3, Kevin Harvey3, Olga V. Volpert4, David N. Brindley5, Joe G. N. Garcia6
1Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
2Indiana University School of MedicineIndianapolisIndiana46202USA
3Methodist Research Institute Indianapolis Indiana 46202 USA
4Northwestern University Medical School, Chicago, Illinois 60611, USA
5University of Alberta Edmonton Alberta T6H 5M3 Canada
6Johns Hopkins University Baltimore Maryland 21205 USA

Tóm tắt

ABSTRACT Recent studies have identified factors responsible for angiogenesis within developing tumors, but mediators of vessel formation at sites of trauma, injury, and wound healing are not clearly established. Here we show that sphingosine 1‐phosphate (S1P) released by platelets during blood clotting is a potent, specific, and selective endothelial cell chemoattractant that accounts for most of the strong endothelial cell chemotactic activity of blood serum, an activity that is markedly diminished in plasma. Preincubation of endothelial cells with pertussis toxin inhibited this effect of S1P, demonstrating the involvement of a Gαi‐coupled receptor. After S1P‐induced migration, endothelial cells proliferated avidly and differentiated forming multicellular structures suggestive of early blood vessel formation. S1P was strikingly effective in enhancing the ability of fibroblast growth factor to induce angiogenesis in the avascular mouse cornea. Our results show that blood coagulation initiates endothelial cell angiogenic responses through the release of S1P, a potent endothelial cell chemoattractant that exerts its effects by activating a receptor‐dependent process.—English, D., Welch, Z., Kovala, A. T., Harvey, K., Volpert, O. V., Brindley, D. N., Garcia, J. G. N. Sphingosine 1‐phosphate released from platelets during clotting accounts for the potent endothelial cell chemotactic activity of blood serum and provides a novel link between hemostasis and angiogenesis. FASEB J. 14, 2255–2265 (2000)

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Tài liệu tham khảo

10.1038/nm0195-27

10.1056/NEJM199512283332608

10.1016/S0021-9258(19)49853-0

10.1093/jnci/86.5.356

10.1016/S0022-2143(98)90004-X

10.1093/jnci/85.2.121

10.1172/JCI119740

10.1038/367576a0

Nissen N. N., 1998, Vascular endothelial growth factor mediated angiogenic activity during the proliferative phase of wound healing, Am. J. Pathol., 152, 1445

10.1038/386671a0

10.1016/S0021-9258(18)47116-5

10.1007/BF02644769

10.1038/362841a0

10.1042/bj2910677

10.1182/blood.V86.1.193.bloodjournal861193

10.1016/0005-2760(89)90112-4

10.1016/S0955-0674(97)80059-2

10.1111/j.1749-6632.1998.tb09658.x

10.1016/S0005-2760(97)00085-4

10.1126/science.279.5356.1552

Kenyon B., 1996, A model of angiogenesis in the mouse cornea, Invest. Opthal., 37, 1625

10.1038/71517

Passaniti A., 1992, A simple, quantitative method for assessing angiogenesis and antiangiogenic agents using reconstituted basement membrane, heparin, and fibroblast growth factor, Lab. Invest., 67, 519

10.1038/sj.onc.1200987

10.1016/S0006-291X(05)81427-5

10.1016/S0021-9258(19)36618-9

10.1074/jbc.274.50.35343

10.1016/S0092-8674(00)81661-X

10.1089/152581699319795

10.1016/S0021-9258(19)38849-0

10.1042/bj3300605