Spectrophotometric Determination of Tranexamic Acid in Pharmaceutical Bulk and Dosage Forms

Analytical Sciences - Tập 21 - Trang 1133-1136 - 2005
Tariq Mahmood Ansari1, Asad Raza1, Atta-ur Rehman2
1Department of Chemistry, Bahauddin Zakariya University, Multan, Pakistan
2BioFine Pharmaceuticals (Pvt.) Ltd., Multan, Pakistan

Tóm tắt

In this study, a simple, fast, accurate and sensitive spectrophotometric method has been developed for the determination of tranexamic acid in bulk and pharmaceutical preparations. The method is based on the reaction of ninhydrin with the primary amino group of tranexamic acid in the basic medium at pH 8.0. The reaction produces a bluish-purple color which absorbs maximally at 565 nm. Beer’s law was obeyed in the range of 3 - 40 μg ml-1 with molar absorptivity of 5.093 × 103 L mol-1 cm-1. The effects of various factors such as temperature, heating time, concentration of reagent, color stability and interferences were investigated to optimize the procedure. The results have been validated analytically and statistically. The proposed method has been applied for the determination of tranexamic acid in bulk and pharmaceutical preparations with good results.

Tài liệu tham khảo

A. R. Gennaro (ed.), “The Science and Pratice of Pharmacy”, 19th ed., 1995, Mack Publishing Company, Easton.

C. J. Dunn and K. L. Goa, Drugs, 1999, 57, 1005.

M. S. Rizk, S. S. Toubar, M. A. Sultan, and S. H. Assaad, Mikrochim. Acta, 2003, 143, 281.

A. M. Wahbi, E. A. Lotfi, and H. Y. Aboul-Enein, Talanta, 1984, 31, 77.

N. Buyuktimkin and S. Buyuktimkin, Acta Pharm. Turc., 1985, 27, 78.

S. Atmaca, Acta Pharm. Turc., 1989, 31, 115.

M. Aboul-Enein and Y. Hassan, Int. J. Environ. Anal. Chem., 1984, 19, 19.

S. Nojiri, S. Uehara, T. Hagiwara, and M. Nishijima, Tokyo-toritsu Eisei Kenkyusho Kenkyu Nenpo, 1995, 46, 58.

K. Inui, S. Doi, Y. Nakai, and K. Yamada, Iyakuhin Kenkyu, 1995, 26, 398.

P. M. Elworthy, S. A. Tsementzis, D. Westhead, and E. R. Hitchcock, J. Chromatogr., 1985, 343, 109.

K. Matsubayashi, C. Kojima, and H. Tachizawa, J. Chromatogr., 1988, 433, 225.

Q. Chang, O. Q. P. Yin, and M. S. S. Chow, J. Chromatogr., B: Anal. Technol. Biomed. Life Sci., 2004, 805, 275.

A. Shalaby, Chin. Pharm. J. [Taipei], 1997, 49, 229.

S. Uehara, T. Hagiwara, M. Takahashi, K. Kamata, K. Nakayama, K. Akiyama, and Y. Naoi, Iyakuhin Kenkyu, 1987, 18, 142.

G. Iskender and S. Atmaca, Pharmazie, 1988, 43, 290.

F. Fritz, “Spot Tests in Organic Analysis”, 7th ed., 1975, Elsevier Publishing Company, Amsterdam.

H. E. Abdellatef and H. M. Khalil, J. Pharm. Biomed. Anal., 2003, 31, 209.

S. Ruhemann, Trans. Chem. Soc., 1910, 97, 2025.

“British Pharmacopoeia”, 2003, Her Majesty’s Stationery Office, London.

Thông tin
Thông tin xuất bản

Analytical Sciences

Tập 21

1133-1136

Thông tin tác giả