Species-specific PCR discrimination of species of the calanoid copepod Pseudocalanus, P. acuspes and P. elongatus, in the Baltic and North Seas

Hydrobiologia - Tập 652 - Trang 289-297 - 2010
Sabine Grabbert1, Jasmin Renz2, Hans-Jürgen Hirche1, Ann Bucklin3
1Alfred Wegener Institute for Polar and Marine Research, Bremerhaven, Germany
2Research Institute Senckenberg, German Centre for Marine Biodiversity Research, Hamburg, Germany
3Department of Marine Sciences, University of Connecticut, Groton, USA

Tóm tắt

Two species of the calanoid copepod Pseudocalanus, P. acuspes and P. elongatus, have been reported from the Baltic and North Seas. Morphometric analysis based on Frost (Can J Zool 67:525–551, 1989) did not identify the species in samples collected during the German GLOBEC program; the majority of measurements fell outside reported ranges for Baltic and North Sea specimens. A multiplexed species-specific PCR (SS-PCR) protocol was designed from a ~700 bp sequence of mitochondrial cytochrome oxidase I (mtCOI), with primers designed to amplify regions of different sizes for the two species to allow detection by gel electrophoresis. The accuracy and reliability of the multiplexed SS-PCR reaction was confirmed by sequencing the mtCOI region. Preliminary SS-PCR analysis of geographic distributions of the two species indicated that P. acuspes occurred exclusively at sampling sites in the Baltic Sea, whereas P. elongatus was found in the southern North Sea and at one site in the Baltic. The SS-PCR protocol can be used for routine identification of the two species for studies of population, community and ecosystem dynamics.

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Hydrobiologia

Tập 652

289-297

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