Species delimitation in Cladonia (Ascomycota): a challenge to the DNA barcoding philosophy

Molecular Ecology Resources - Tập 13 Số 6 - Trang 1058-1068 - 2013
Raquel Pino‐Bodas1, María P. Martín2, Ana Rosa Burgaz1, H. Thorsten Lumbsch3
1Departamento Biología Vegetal 1 Facultad de Biología Universidad Complutense de Madrid E‐28040 Madrid Spain
2Departamento de Micología Real Jardín Botánico RJB‐CSIC E‐28014 Madrid Spain
3Department of Botany, The Field Museum, 1400 S. Lake Shore Drive, Chicago, IL 60605, USA

Tóm tắt

AbstractThe lichen‐forming fungal genus Cladonia is species‐rich with approximately 500 described species. The accepted barcode for fungi (ITS rDNA) often fails in identifying Cladonia spp. In order to find other markers that, in combination with the ITS rDNA region can be used for species identification in Cladonia, we studied the loci IGS rDNA, ef1α, rpb2 and cox1. A total of 782 sequences from 36 species have been analyzed. PCR amplification success rate, intraspecific and interspecific genetic distance variation, calculated using the K2P model, and the correct identification percentage (PCI) were taken into account to assess possible barcode regions. The marker showing the least intraspecific genetic distance range was cox1, followed by ITS rDNA and ef1α. Of the five studied markers only cox1 showed a barcoding gap. The rpb2 locus showed the highest PCI values, but it was the most difficult to amplify. The highest correct identification rates using blast method were obtained with rpb2.

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Molecular Ecology Resources

Tập 13 Số 6

1058-1068

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