Spatial control of neuronal cell attachment and differentiation on covalently patterned laminin oligopeptide substrates

International Journal of Developmental Neuroscience - Tập 12 - Trang 725-735 - 1994
John P. Ranieri1, Ravi Bellamkonda1, Evan J. Bekos2, Joseph A. Gardella2, Hans J. Mathieu3, Laurence Ruiz3, Patrick Aebischer1
1Division of Surgical Research, Centre Hospitalier Universitaire Vaudois, Lausanne University Medical School, 1011 Lausanne, Switzerland
2Department of Chemistry, State University of New York at Buffalo, Buffalo, NY 14214-3094, U.S.A.
3Department of Material Science, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland

Tóm tắt

AbstractThe spatial control of neuronal cell attachment and differentiation via specific receptor mediated interactions, may provide an effective means for the in vitro reconstruction of neuronal cell architecture. In this study, receptor‐specific oligopeptide sequences derived from the extracellular matrix (ECM) molecule laminin, a potent neural cell attachment and differentiation promoter were covalently bound on fluorinated ethylene propylene (FEP) films. The degree of receptor‐specific cell attachment and the ability to spatially control neurite outgrowth by covalently patterning the oligopeptide sequences on the FEP film surface were assessed.FEP films were first chemically activated with a Radio Frequency Glow Discharge (RFGD) process that covalently replaces the surface fluorine atoms with reactive hydroxyl groups. Oligopeptides containing the YIGSR sequence from the B1 chain of laminin and the water soluble oligopeptide containing the IKVAV sequence (CSRARKQAASIKVAVSADR) from the A chain were covalently bound to the hydroxylated FEP films. Electron Spectroscopy for Chemical Analysis (ESCA) verified the covalent attachment of the oligopeptides to the material surface.The degree of receptor mediated NG108‐15 cell attachment on immobilized CDPGYIGSR films was determined using competitive binding media. A 78% reduction in cell attachment was observed on films containing CDPGYIGSR in the cell plating medium. Only a 23% reduction in cell attachment was noted on films plated in medium containing a mock CDPGYIGSK sequence. FEP films immobilized with the IKVAV oligopeptide sequence were shown to mediate PC12 cell attachment and a competitive binding medium also significantly attenuated cell attachment on the immobilized films.The spatial patterning of these oligopeptide sequences to the FEP surface was shown to localize cell attachment and neurite extension on the patterned pathways. The surrounding unmodified FEP surface was inhibitory in serum containing medium and prevented cellular interactions outside the oligopeptide modifications. The spatial immobilization of laminin oligopeptides on FEP films provides a means to organize the attachment and differentiation of neuronal cells in a receptor‐specific manner.

Tài liệu tham khảo

10.1096/fasebj.4.11.2199285 BekosE. J. BrightF. V. RanieriJ. P. AebischerP.andGardellaJ. A.Jr(in press)IR spectroscopy analysis of bovine serum albumin's adsorption conformation on polymer surfaces.Langmuir. 10.1016/0014-4827(92)90157-4 10.1007/3-540-08124-0_3 10.1016/0896-6273(91)90345-Z 10.1016/0955-0674(92)90100-Q 10.1126/science.2020853 10.1016/0304-3940(91)90724-8 10.1016/0955-0674(92)90099-X 10.1016/0092-8674(87)90707-0 10.1016/0076-6879(87)35068-2 10.1038/nbt0691-568 10.1016/0006-8993(91)90356-Z 10.1002/jnr.490280407 10.1523/JNEUROSCI.08-11-04098.1988 10.1002/jcb.240270402 10.1073/pnas.85.4.1282 10.1523/JNEUROSCI.12-03-00818.1992 10.1146/annurev.cb.03.110187.000421 10.1016/0003-2697(90)90459-M 10.1038/350068a0 10.1038/309030a0 10.1073/pnas.81.19.5985 10.1002/jbm.820270711 RanieriJ. P. BellamkondaR. BekosE. J. VargoT. G. GardellaJrJ. A.andAebischerP.(in press)Neuronal cell attachment to fluorinated ethylene propylene films with covalently immobilized laminin oligopeptides YIGSR and IKVAV.J. Biomed. Mater. Res. 10.1007/BF02363290 10.1126/science.2821619 10.1146/annurev.ne.16.030193.003025 10.1159/000111909 10.1016/0006-291X(89)92384-X 10.1016/S0014-4886(05)80013-5 Tashiro K.I., 1989, A synthetic peptide containing the IKVAV sequence from the A chain of laminin mediates cell attachment, migration, and neurite outgrowth, J. biol. Chem., 264, 16,174, 10.1016/S0021-9258(18)71604-9 10.1016/S0232-1513(86)80002-0 10.1159/000111908 10.1002/jbm.820260302 10.1242/dev.101.4.909 10.1126/science.7684161 10.1002/pola.1991.080290412 T.J.Vargo E.J.Bekos Y.S.Kim‐Jo J.P.Ranieri R.Bellamkonda P.Aebischer D.E.Margevich P.M.Thompson F.V.Bright J.A.GardellaJr..Synthesis and characterization of fluoropolymeric substrata with immobilized minimal peptide sequences.J. Biomed. Mater. Res.1995
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International Journal of Developmental Neuroscience

Tập 12

725-735

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