Sources of variability in data from a lacl transgenic mouse mutation assay

Environmental and Molecular Mutagenesis - Tập 23 Số 1 - Trang 17-31 - 1994
Walter W. Piegorsch1, Ann‐Marie C. Lockhart2, Barry H. Margolin3, Kenneth R. Tindall4, Nancy J. Gorelick5, Jay M. Short6, Gregory J. Carr5, Elizabeth I. Thompson5, Michael D. Shelby4
1University of South Carolina, Columbia
2Computer Sciences Corp., Research Triangle Park
3University of North Carolina, Chapel Hill, North Carolina
4National Institute of Environmental Health Sciences, Research Triangle Park
5Procter & Gamble Co., Cincinnati, Ohio,
6Stratagene, La Jolla, California

Tóm tắt

AbstractExperimental features of a transgenic mouse mutation assay based on a lacl target transgene from Escherichia coli are considered in detail. Sources of variability in the experimental protocol that can affect the statistical nature of the observations are examined with the goal of identifying sources of excess variation in the observed mutant fractions. The sources include plate‐to‐plate (within packages), package‐to‐package (within animals), and animal‐to‐animal (within study) variability. Data from two laboratories are evaluated, using various statistical methods to identify excess variability. Results suggest only scattered patterns of excess variability, except possibly in those cases where genomic DNA from test animals is stored for extended periods (e.g., > 90 days) after isolation from tissues. Further study is encouraged to examine the validity and implications of this time/storage‐related effect.

Từ khóa


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Environmental and Molecular Mutagenesis

Tập 23 Số 1

17-31

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