Soluble Interleukin‐6 Receptor Is Released from Receptor‐bearing Cell Linesin vitro

Wiley - Tập 83 Số 4 - Trang 373-378 - 1992
Toshiharu Nakajima1,2, Shinji Yamamoto2, Ming Cheng2, Kiyoshi Yasukawa3, Toshio Hirano4, Tadamitsu Kishimoto5, Tohru Tokunaga2, Mitsuo Honda2
1Institute of Medical Science, St. Marianna University, 2-16-l Sugao, Miyamae-ku, Kanagawa 216.
2Laboratory of Immunology, AIDS Research Center, National Institute of Health, 2-10-35 Kamiosaki, Shinagawa-ku, Tokyo 141
3Biotechnology Research Laboratory, Tosoh Corporation, 2743-1 Hayakawa, Ayase, Kanagawa 252
4Division of Molecular Oncology, Biomedical Research Center, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565
5Department of Internal Medicine, Osaka University Medical School, 1-1-50 Fukushima, Fukushima-ku, Osaka 553

Tóm tắt

Soluble interleukin‐6 receptor (sIL‐6R) was found to be spontaneously released from human myeloma cell line U266 cells into culture supernatant, and was quantitatively measured with a fluorescence sandwich enzyme‐linked immunosorbent assay employing antibodies specific to IL‐6R. The supernatant IL‐6R was generated only from IL‐6R‐positive cell lines; myeloma cell lines RPMI8226 and RPMI1788, and myelomonocytic cell lines U937, THP‐1, and HL‐60. In contrast, it was not released from the IL‐6R‐negative cells; T cell line MoIt‐4 and Burkitt lympboma cell line Raji. SDS‐PAGE analysis of the soluble IL‐6R from U266 cells suggested a molecular weight of approximately 50‐55 kDa, 25–30 kDa smaller than the mature cell surface receptor. These results suggest that the generation of soluble IL‐6R may be a maker of myeloma cells and myelomonocytic cells.

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Wiley

Tập 83 Số 4

373-378

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