Soft Tissue Augmentation Using Silk Gels: An In Vitro and In Vivo Study

Journal of Periodontology - Tập 80 Số 11 - Trang 1852-1858 - 2009
Etienne Olivier1,2, Aurore Schneider3,1, Jonathan A. Kluge4, Claire Bellemin‐Laponnaz1, Camille Polidori1, Gary G. Leisk4,5, David L. Kaplan4, William P. Meehan4,1, Christophe Egles4,1
1Division of Cancer Biology and Tissue Engineering, Department of Oral and Maxillofacial Pathology, School of Dental Medicine, Tufts University, Boston, MA.
2School of Dental Medicine, Department of Prosthodontics, Louis Pasteur University, Strasbourg, France.
3Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, MA.
4Department of Biomedical Engineering, Tufts University, Medford, MA
5Department of Mechanical Engineering, Tufts University, Medford, MA.

Tóm tắt

Background: Restoration of a three‐dimensional shape with soft tissue augmentation is a challenge for surgical reconstruction and esthetic improvement of intraoral mucosa and perioral skin tissues. A connective tissue graft or free gingival graft, classically used for such indications, requires a donor site, which may lead to various clinical complications.Methods: In this article, a new three‐dimensional scaffold made of silk fibroin that could be of great interest for these indications was studied. Mechanical tests were conducted to characterize the physical properties of the materials. The biocompatibility of such scaffolds was positively assessed in vitro using a combination of immunostaining, 5‐bromo‐2′‐deoxyuridine proliferation assays, and histologic staining. Finally, the shaped material was grafted subcutaneously in nude mice for a long‐time implantation study.Results: Human fibroblasts embedded in this material had a survival rate up to 68.4% and were able to proliferate and synthesize proteins. One month after subcutaneous implantation, the three‐dimensional soft tissue augmentation was stable, and histologic analysis revealed revascularization of the area through the biomaterial. A mild inflammatory reaction disappeared after 12 weeks.Conclusion: The results indicate that silk‐gel material was able to create a lasting three‐dimensional soft tissue augmentation and is a promising biomaterial for periodontal and maxillofacial therapies, either as a scaffold for cells or alone as a biomaterial.

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Tài liệu tham khảo

Evian CI, 2003, Soft tissue augmentation for implant dentistry, Compend Contin Educ Dent, 24, 195

10.1001/archotol.124.11.1219

10.1111/j.1600-0757.1996.tb00185.x

Seibert JS, 1993, Treatment of moderate localized alveolar ridge defects. Preventive and reconstructive concepts in therapy, Dent Clin North Am, 37, 265, 10.1016/S0011-8532(22)00280-4

Aghaloo TL, 2007, Which hard tissue augmentation techniques are the most successful in furnishing bony support for implant placement?, Int J Oral Maxillofac Implants, 22, 49

10.1016/S0094-1298(20)32754-1

Cohen ES, 1994, Ridge augmentation utilizing the subepithelial connective tissue graft: Case reports, Pract Periodontics Aesthet Dent, 6, 47

10.1902/annals.2003.8.1.303

10.1902/jop.2006.050296

10.1097/00006534-199003000-00007

10.1053/joms.2002.31229

10.1111/j.1524-4725.1995.tb00252.x

10.1902/jop.2005.76.11.1814

10.1111/j.1600-0560.2007.00853.x

10.1089/ten.2004.10.1672

10.1177/154405910708600203

10.1016/j.tibtech.2008.02.006

10.1016/S0142-9612(02)00353-8

10.1038/nature01809

10.1016/j.jmb.2003.10.043

10.1002/1097-4636(200101)54:1<139::AID-JBM17>3.0.CO;2-7

10.1016/j.biomaterials.2007.11.003

Winter GD, 1972, Epidermal regeneration in the domestic pig, 71

Seibert JS, 1983, Reconstruction of deformed, partially edentulous ridges, using full thickness onlay grafts. Part I. Technique and wound healing, Compend Contin Educ Dent, 4, 437

Studer S, 1997, Adjustment of localized alveolar ridge defects by soft tissue transplantation to improve mucogingival esthetics: A proposal for clinical classification and an evaluation of procedures, Quintessence Int, 28, 785

Strietzel FP, 2001, Risks and complications of membrane‐guided bone regeneration. Retrospective analysis [in German], Oral and Maxillofacial Surgery, 5, 28

10.5005/jcdp-2-3-11

10.1007/BF02344783

10.1016/j.biomaterials.2004.09.020

10.1021/bm034327e

10.1016/j.biomaterials.2008.05.002

Hanke CW, 1997, Dermal filler substances, 217

10.1038/418741a

10.1016/S0142-9612(02)00156-4

10.1071/CH05170

10.4012/dmj.4.47

10.1021/bm025581u

10.1021/bm0343287

10.1016/j.biomaterials.2004.11.013

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Journal of Periodontology

Tập 80 Số 11

1852-1858

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