Single-Cell Quantification of mRNA Expression in The Human Brain

Scientific Reports - Tập 9 Số 1
Sarah Jolly1, Verena Lang1, Viktor H. Koelzer2, Carlo Sala Frigerio3, Lorenza Magno1, Patricia C. Salinas4, Paul Whiting3, Ernest Palomer4
1ARUK-UCL Drug Discovery Institute, University College London, London, United Kingdom
2Department of Pathology and Molecular Pathology, University and University Hospital Zurich, Zurich, Switzerland
3UK Dementia Research Institute, University College London, London, United Kingdom
4Department of Cell and Developmental Biology, University College London, London, United Kingdom

Tóm tắt

AbstractRNA analysis at the cellular resolution in the human brain is challenging. Here, we describe an optimised approach for detecting single RNA transcripts in a cell-type specific manner in frozen human brain tissue using multiplexed fluorescent RNAscope probes. We developed a new robust analytical approach for RNAscope quantification. Our method shows that low RNA integrity does not significantly affect RNAscope signal, recapitulates bulk RNA analysis and provides spatial context to transcriptomic analysis of human post-mortem brain at single-cell resolution. In summary, our optimised method allows the usage of frozen human samples from brain banks to perform quantitative RNAscope analysis.

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Scientific Reports

Tập 9 Số 1

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